Early Developmental Zebrafish Embryo Extract to Modulate Senescence in Multisource Human Mesenchymal Stem Cells
Stem cells undergo senescence both in vivo, contributing to the progressive decline in self-healing mechanisms, and in vitro during prolonged expansion. Here, we show that an early developmental zebrafish embryo extract (ZF1) could act as a modulator of senescence in human mesenchymal stem cells (hM...
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doaj-b92abba868124578b125d52212c2a8622020-11-25T01:51:15ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-05-012011264610.3390/ijms20112646ijms20112646Early Developmental Zebrafish Embryo Extract to Modulate Senescence in Multisource Human Mesenchymal Stem CellsFederica Facchin0Francesco Alviano1Silvia Canaider2Eva Bianconi3Martina Rossi4Laura Bonsi5Raffaella Casadei6Pier Mario Biava7Carlo Ventura8Department of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, Via Massarenti 9, 40138 Bologna, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, Via Massarenti 9, 40138 Bologna, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, Via Massarenti 9, 40138 Bologna, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, Via Massarenti 9, 40138 Bologna, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, Via Massarenti 9, 40138 Bologna, ItalyDepartment of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, Via Massarenti 9, 40138 Bologna, ItalyDepartment for Life Quality Studies (QuVi), University of Bologna, Corso D’Augusto 237, 47921 Rimini, ItalyScientific Institute of Research and Care Multimedica, Via Milanese 300, 20099 Sesto San Giovanni (Milano), ItalyDepartment of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, Via Massarenti 9, 40138 Bologna, ItalyStem cells undergo senescence both in vivo, contributing to the progressive decline in self-healing mechanisms, and in vitro during prolonged expansion. Here, we show that an early developmental zebrafish embryo extract (ZF1) could act as a modulator of senescence in human mesenchymal stem cells (hMSCs) isolated from both adult tissues, including adipose tissue (hASCs), bone marrow (hBM-MSCs), dental pulp (hDP-MSCs), and a perinatal tissue such as the Wharton’s Jelly (hWJ-MSCs). In all the investigated hMSCs, ZF1 decreased senescence-associated β-galactosidase (SA β-gal) activity and enhanced the transcription of <i>TERT</i>, encoding the catalytic telomerase core. In addition, it was associated, only in hASCs, with a transcriptional induction of <i>BMI1</i>, a pleiotropic repressor of senescence. In hBM-MSCs, hDP-MSCs, and hWJ-MSCs, <i>TERT</i> over-expression was concomitant with a down-regulation of two repressors of <i>TERT</i>, <i>TP53</i> (<i>p53</i>), and <i>CDKN1A</i> (<i>p21</i>). Furthermore, ZF1 increased the natural ability of hASCs to perform adipogenesis. These results indicate the chance of using ZF1 to modulate stem cell senescence in a source-related manner, to be potentially used as a tool to affect stem cell senescence in vitro. In addition, its anti-senescence action could also set the basis for future in vivo approaches promoting tissue rejuvenation bypassing stem cell transplantation.https://www.mdpi.com/1422-0067/20/11/2646stem cellssenescencezebrafish embryo extractsenescence-associated β-galactosidase activityadipogenesis<i>TERT</i><i>BMI1</i><i>p53</i><i>p21</i><i>p16</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Federica Facchin Francesco Alviano Silvia Canaider Eva Bianconi Martina Rossi Laura Bonsi Raffaella Casadei Pier Mario Biava Carlo Ventura |
spellingShingle |
Federica Facchin Francesco Alviano Silvia Canaider Eva Bianconi Martina Rossi Laura Bonsi Raffaella Casadei Pier Mario Biava Carlo Ventura Early Developmental Zebrafish Embryo Extract to Modulate Senescence in Multisource Human Mesenchymal Stem Cells International Journal of Molecular Sciences stem cells senescence zebrafish embryo extract senescence-associated β-galactosidase activity adipogenesis <i>TERT</i> <i>BMI1</i> <i>p53</i> <i>p21</i> <i>p16</i> |
author_facet |
Federica Facchin Francesco Alviano Silvia Canaider Eva Bianconi Martina Rossi Laura Bonsi Raffaella Casadei Pier Mario Biava Carlo Ventura |
author_sort |
Federica Facchin |
title |
Early Developmental Zebrafish Embryo Extract to Modulate Senescence in Multisource Human Mesenchymal Stem Cells |
title_short |
Early Developmental Zebrafish Embryo Extract to Modulate Senescence in Multisource Human Mesenchymal Stem Cells |
title_full |
Early Developmental Zebrafish Embryo Extract to Modulate Senescence in Multisource Human Mesenchymal Stem Cells |
title_fullStr |
Early Developmental Zebrafish Embryo Extract to Modulate Senescence in Multisource Human Mesenchymal Stem Cells |
title_full_unstemmed |
Early Developmental Zebrafish Embryo Extract to Modulate Senescence in Multisource Human Mesenchymal Stem Cells |
title_sort |
early developmental zebrafish embryo extract to modulate senescence in multisource human mesenchymal stem cells |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2019-05-01 |
description |
Stem cells undergo senescence both in vivo, contributing to the progressive decline in self-healing mechanisms, and in vitro during prolonged expansion. Here, we show that an early developmental zebrafish embryo extract (ZF1) could act as a modulator of senescence in human mesenchymal stem cells (hMSCs) isolated from both adult tissues, including adipose tissue (hASCs), bone marrow (hBM-MSCs), dental pulp (hDP-MSCs), and a perinatal tissue such as the Wharton’s Jelly (hWJ-MSCs). In all the investigated hMSCs, ZF1 decreased senescence-associated β-galactosidase (SA β-gal) activity and enhanced the transcription of <i>TERT</i>, encoding the catalytic telomerase core. In addition, it was associated, only in hASCs, with a transcriptional induction of <i>BMI1</i>, a pleiotropic repressor of senescence. In hBM-MSCs, hDP-MSCs, and hWJ-MSCs, <i>TERT</i> over-expression was concomitant with a down-regulation of two repressors of <i>TERT</i>, <i>TP53</i> (<i>p53</i>), and <i>CDKN1A</i> (<i>p21</i>). Furthermore, ZF1 increased the natural ability of hASCs to perform adipogenesis. These results indicate the chance of using ZF1 to modulate stem cell senescence in a source-related manner, to be potentially used as a tool to affect stem cell senescence in vitro. In addition, its anti-senescence action could also set the basis for future in vivo approaches promoting tissue rejuvenation bypassing stem cell transplantation. |
topic |
stem cells senescence zebrafish embryo extract senescence-associated β-galactosidase activity adipogenesis <i>TERT</i> <i>BMI1</i> <i>p53</i> <i>p21</i> <i>p16</i> |
url |
https://www.mdpi.com/1422-0067/20/11/2646 |
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