A novel ultra high-throughput 16S rRNA gene amplicon sequencing library preparation method for the Illumina HiSeq platform

A novel ultra high-throughput 16S rRNA gene amplicon sequencing library preparation method for the Illumina HiSeq platform

Abstract Background Advances in sequencing technologies and bioinformatics have made the analysis of microbial communities almost routine. Nonetheless, the need remains to improve on the techniques used for gathering such data, including increasing throughput while lowering cost and benchmarking the...

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Bibliographic Details
Main Authors: Eric J. de Muinck, Pål Trosvik, Gregor D. Gilfillan, Johannes R. Hov, Arvind Y. M. Sundaram
Format: Article
Language:English
Published: BMC 2017-07-01
Series:Microbiome
Subjects:
16S rRNA gene amplicon sequencing
Illumina library preparation
Indexed PCR
Mock community
Environmental sequencing
Benchmarking
Online Access:http://link.springer.com/article/10.1186/s40168-017-0279-1

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http://link.springer.com/article/10.1186/s40168-017-0279-1

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