Rapid, Reproducible, Quantifiable NMR Metabolomics: Methanol and Methanol: Chloroform Precipitation for Removal of Macromolecules in Serum and Whole Blood

Rapid, Reproducible, Quantifiable NMR Metabolomics: Methanol and Methanol: Chloroform Precipitation for Removal of Macromolecules in Serum and Whole Blood

Background: Though blood is an excellent biofluid for metabolomics, proteins and lipids present in blood can interfere with 1<span style="font-variant: small-caps;">d</span>-<sup>1</sup>H NMR spectra and disrupt quantification of metabolites. Here, we present effect...

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Bibliographic Details
Main Authors: Cora E. McHugh, Thomas L. Flott, Casey R. Schooff, Zyad Smiley, Michael A. Puskarich, Daniel D. Myers, John G. Younger, Alan E. Jones, Kathleen A. Stringer
Format: Article
Language:English
Published: MDPI AG 2018-12-01
Series:Metabolites
Subjects:
pharmacometabolomics
extraction
ultrafiltration
1<span style="font-variant: small-caps">d</span>-<sup>1</sup>H NMR
quantitative analysis
preanalytical processing
Online Access:https://www.mdpi.com/2218-1989/8/4/93

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https://www.mdpi.com/2218-1989/8/4/93

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