Purification, kinetic characterization, and site-directed mutagenesis of <em>Methanothermobacter thermautotrophicus</em> RFAP Synthase Produced in <em>Escherichia coli</em>

Purification, kinetic characterization, and site-directed mutagenesis of <em>Methanothermobacter thermautotrophicus</em> RFAP Synthase Produced in <em>Escherichia coli</em>

Methane-producing archaea are among a select group of microorganisms that utilize tetrahydromethanopterin (H<sub>4</sub>MPT) as a one-carbon carrier instead of tetrahydrofolate. In H<sub>4</sub>MPT biosynthesis, β-ribofuranosylaminobenzene 5’-phosphate (RFAP) synthase catalyz...

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Bibliographic Details
Main Authors: Matthew E. Bechard, Payam Farahani, Dina Greene, Anna Pham, Andrew Orry, Madeline E. Rasche
Format: Article
Language:English
Published: AIMS Press 2019-07-01
Series:AIMS Microbiology
Subjects:
methanogenesis
methanopterin
rfap synthase
site-directed mutagenesis
substrate binding
Online Access:https://www.aimspress.com/article/10.3934/microbiol.2019.3.186/fulltext.html

Internet

https://www.aimspress.com/article/10.3934/microbiol.2019.3.186/fulltext.html

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