Purification, kinetic characterization, and site-directed mutagenesis of <em>Methanothermobacter thermautotrophicus</em> RFAP Synthase Produced in <em>Escherichia coli</em>
Methane-producing archaea are among a select group of microorganisms that utilize tetrahydromethanopterin (H<sub>4</sub>MPT) as a one-carbon carrier instead of tetrahydrofolate. In H<sub>4</sub>MPT biosynthesis, β-ribofuranosylaminobenzene 5’-phosphate (RFAP) synthase catalyz...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
AIMS Press
2019-07-01
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Series: | AIMS Microbiology |
Subjects: | |
Online Access: | https://www.aimspress.com/article/10.3934/microbiol.2019.3.186/fulltext.html |