Optimization of flotation, DNA extraction and PCR methods for detection of Toxoplasma gondii oocysts in cat faeces

Optimization of flotation, DNA extraction and PCR methods for detection of Toxoplasma gondii oocysts in cat faeces

Introduction and objective The aim of the study was to compare the effectiveness of selected oocysts concentration methods, DNA extraction protocols and PCR assays targeting the B1 gene, for the development of procedures which would be effective and useful in laboratory practice for the detection of...

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Bibliographic Details
Main Authors: Jacek Sroka, Jacek Karamon, Jacek Dutkiewicz, Angelina Wójcik-Fatla, Tomasz Cencek
Format: Article
Language:English
Published: Institute of Rural Health 2018-12-01
Series:Annals of Agricultural and Environmental Medicine
Subjects:
flotation
Toxoplasma gondii
faeces
Real time PCR
nested PCR
cats
oocysts detection
Online Access:http://www.journalssystem.com/aaem/Optimization-of-flotation-DNA-extraction-and-PCR-methods-for-detection-of-Toxoplasma,97402,0,2.html

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http://www.journalssystem.com/aaem/Optimization-of-flotation-DNA-extraction-and-PCR-methods-for-detection-of-Toxoplasma,97402,0,2.html

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