Optimization of Fluorescent Labeling for In Vivo Nanoimaging of Sarcomeres in the Mouse Heart

The present study was conducted to systematically investigate the optimal viral titer as well as the volume of the adenovirus vector (ADV) that expresses α-actinin-AcGFP in the Z-disks of myocytes in the left ventricle (LV) of mice. An injection of 10 μL ADV at viral titers of 2 to 4 × 1011 viral pa...

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Bibliographic Details
Main Authors: Fuyu Kobirumaki-Shimozawa, Togo Shimozawa, Kotaro Oyama, Yasuharu Kushida, Takako Terui, Shin’ichi Ishiwata, Norio Fukuda
Format: Article
Language:English
Published: Hindawi Limited 2018-01-01
Series:BioMed Research International
Online Access:http://dx.doi.org/10.1155/2018/4349170