A CRISPR Interference Screen of Essential Genes Reveals that Proteasome Regulation Dictates Acetic Acid Tolerance in <named-content content-type="genus-species">Saccharomyces cerevisiae</named-content>

A CRISPR Interference Screen of Essential Genes Reveals that Proteasome Regulation Dictates Acetic Acid Tolerance in <named-content content-type="genus-species">Saccharomyces cerevisiae</named-content>

ABSTRACT CRISPR interference (CRISPRi) is a powerful tool to study cellular physiology under different growth conditions, and this technology provides a means for screening changed expression of essential genes. In this study, a Saccharomyces cerevisiae CRISPRi library was screened for growth in med...

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Bibliographic Details
Main Authors: Vaskar Mukherjee, Ulrika Lind, Robert P. St. Onge, Anders Blomberg, Yvonne Nygård
Format: Article
Language:English
Published: American Society for Microbiology 2021-08-01
Series:mSystems
Subjects:
CRISPR interference
yeast
high-throughput screening
acetic acid tolerance
essential genes
transcriptional regulation
Online Access:https://journals.asm.org/doi/10.1128/mSystems.00418-21

Internet

https://journals.asm.org/doi/10.1128/mSystems.00418-21

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