Serum-Free Suspension Culture of MDCK Cells for Production of Influenza H1N1 Vaccines.
Development of serum-free suspension cell culture processes is very important for influenza vaccine production. Previously, we developed a MDCK suspension cell line in a serum-free medium. In the present study, the growth kinetics of suspension MDCK cells and influenza virus production in the serum-...
Main Authors: | , , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2015-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4634975?pdf=render |
id |
doaj-ba1fe293e4614eb18116b88ca2307e93 |
---|---|
record_format |
Article |
spelling |
doaj-ba1fe293e4614eb18116b88ca2307e932020-11-25T00:41:48ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011011e014168610.1371/journal.pone.0141686Serum-Free Suspension Culture of MDCK Cells for Production of Influenza H1N1 Vaccines.Ding HuangWen-Juan PengQian YeXu-Ping LiuLiang ZhaoLi FanKang Xia-HouHan-Jing JiaJian LuoLin-Ting ZhouBei-Bei LiShi-Lei WangWen-Ting XuZe ChenWen-Song TanDevelopment of serum-free suspension cell culture processes is very important for influenza vaccine production. Previously, we developed a MDCK suspension cell line in a serum-free medium. In the present study, the growth kinetics of suspension MDCK cells and influenza virus production in the serum-free medium were investigated, in comparison with those of adherent MDCK cells in both serum-containing and serum-free medium. It was found that the serum-free medium supported the stable subculture and growth of both adherent and suspension cells. In batch culture, for both cell lines, the growth kinetics in the serum-free medium was comparable with those in the serum-containing medium and a commercialized serum-free medium. In the serum-free medium, peak viable cell density (VCD), haemagglutinin (HA) and median tissue culture infective dose (TCID50) titers of the two cell lines reached 4.51×106 cells/mL, 2.94Log10(HAU/50 μL) and 8.49Log10(virions/mL), and 5.97×106 cells/mL, 3.88Log10(HAU/50 μL), and 10.34Log10(virions/mL), respectively. While virus yield of adherent cells in the serum-free medium was similar to that in the serum-containing medium, suspension culture in the serum-free medium showed a higher virus yield than adherent cells in the serum-containing medium and suspension cells in the commercialized serum-free medium. However, the percentage of infectious viruses was lower for suspension culture in the serum-free medium. These results demonstrate the great potential of this suspension MDCK cell line in serum-free medium for influenza vaccine production and further improvements are warranted.http://europepmc.org/articles/PMC4634975?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ding Huang Wen-Juan Peng Qian Ye Xu-Ping Liu Liang Zhao Li Fan Kang Xia-Hou Han-Jing Jia Jian Luo Lin-Ting Zhou Bei-Bei Li Shi-Lei Wang Wen-Ting Xu Ze Chen Wen-Song Tan |
spellingShingle |
Ding Huang Wen-Juan Peng Qian Ye Xu-Ping Liu Liang Zhao Li Fan Kang Xia-Hou Han-Jing Jia Jian Luo Lin-Ting Zhou Bei-Bei Li Shi-Lei Wang Wen-Ting Xu Ze Chen Wen-Song Tan Serum-Free Suspension Culture of MDCK Cells for Production of Influenza H1N1 Vaccines. PLoS ONE |
author_facet |
Ding Huang Wen-Juan Peng Qian Ye Xu-Ping Liu Liang Zhao Li Fan Kang Xia-Hou Han-Jing Jia Jian Luo Lin-Ting Zhou Bei-Bei Li Shi-Lei Wang Wen-Ting Xu Ze Chen Wen-Song Tan |
author_sort |
Ding Huang |
title |
Serum-Free Suspension Culture of MDCK Cells for Production of Influenza H1N1 Vaccines. |
title_short |
Serum-Free Suspension Culture of MDCK Cells for Production of Influenza H1N1 Vaccines. |
title_full |
Serum-Free Suspension Culture of MDCK Cells for Production of Influenza H1N1 Vaccines. |
title_fullStr |
Serum-Free Suspension Culture of MDCK Cells for Production of Influenza H1N1 Vaccines. |
title_full_unstemmed |
Serum-Free Suspension Culture of MDCK Cells for Production of Influenza H1N1 Vaccines. |
title_sort |
serum-free suspension culture of mdck cells for production of influenza h1n1 vaccines. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2015-01-01 |
description |
Development of serum-free suspension cell culture processes is very important for influenza vaccine production. Previously, we developed a MDCK suspension cell line in a serum-free medium. In the present study, the growth kinetics of suspension MDCK cells and influenza virus production in the serum-free medium were investigated, in comparison with those of adherent MDCK cells in both serum-containing and serum-free medium. It was found that the serum-free medium supported the stable subculture and growth of both adherent and suspension cells. In batch culture, for both cell lines, the growth kinetics in the serum-free medium was comparable with those in the serum-containing medium and a commercialized serum-free medium. In the serum-free medium, peak viable cell density (VCD), haemagglutinin (HA) and median tissue culture infective dose (TCID50) titers of the two cell lines reached 4.51×106 cells/mL, 2.94Log10(HAU/50 μL) and 8.49Log10(virions/mL), and 5.97×106 cells/mL, 3.88Log10(HAU/50 μL), and 10.34Log10(virions/mL), respectively. While virus yield of adherent cells in the serum-free medium was similar to that in the serum-containing medium, suspension culture in the serum-free medium showed a higher virus yield than adherent cells in the serum-containing medium and suspension cells in the commercialized serum-free medium. However, the percentage of infectious viruses was lower for suspension culture in the serum-free medium. These results demonstrate the great potential of this suspension MDCK cell line in serum-free medium for influenza vaccine production and further improvements are warranted. |
url |
http://europepmc.org/articles/PMC4634975?pdf=render |
work_keys_str_mv |
AT dinghuang serumfreesuspensioncultureofmdckcellsforproductionofinfluenzah1n1vaccines AT wenjuanpeng serumfreesuspensioncultureofmdckcellsforproductionofinfluenzah1n1vaccines AT qianye serumfreesuspensioncultureofmdckcellsforproductionofinfluenzah1n1vaccines AT xupingliu serumfreesuspensioncultureofmdckcellsforproductionofinfluenzah1n1vaccines AT liangzhao serumfreesuspensioncultureofmdckcellsforproductionofinfluenzah1n1vaccines AT lifan serumfreesuspensioncultureofmdckcellsforproductionofinfluenzah1n1vaccines AT kangxiahou serumfreesuspensioncultureofmdckcellsforproductionofinfluenzah1n1vaccines AT hanjingjia serumfreesuspensioncultureofmdckcellsforproductionofinfluenzah1n1vaccines AT jianluo serumfreesuspensioncultureofmdckcellsforproductionofinfluenzah1n1vaccines AT lintingzhou serumfreesuspensioncultureofmdckcellsforproductionofinfluenzah1n1vaccines AT beibeili serumfreesuspensioncultureofmdckcellsforproductionofinfluenzah1n1vaccines AT shileiwang serumfreesuspensioncultureofmdckcellsforproductionofinfluenzah1n1vaccines AT wentingxu serumfreesuspensioncultureofmdckcellsforproductionofinfluenzah1n1vaccines AT zechen serumfreesuspensioncultureofmdckcellsforproductionofinfluenzah1n1vaccines AT wensongtan serumfreesuspensioncultureofmdckcellsforproductionofinfluenzah1n1vaccines |
_version_ |
1725285533883564032 |