A neutralizing anti-gH/gL monoclonal antibody is protective in the guinea pig model of congenital CMV infection.
Human cytomegalovirus (HCMV) is the most common cause of congenital virus infection. Congenital HCMV infection occurs in 0.2-1% of all births, and causes birth defects and developmental abnormalities, including sensorineural hearing loss and developmental delay. Several key studies have established...
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2014-04-01
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doaj-ba33e7d6b927441a9bc774597b8ee3e52020-11-24T22:09:33ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742014-04-01104e100406010.1371/journal.ppat.1004060A neutralizing anti-gH/gL monoclonal antibody is protective in the guinea pig model of congenital CMV infection.Marcy R AuerbachDonghong YanRajesh VijJo-Anne HongoGerald NakamuraJean-Michel VernesY Gloria MengSamantha LeinPamela ChanJed RossRichard CaranoRong DengNicholas Lewin-KohMin XuBecket FeierbachHuman cytomegalovirus (HCMV) is the most common cause of congenital virus infection. Congenital HCMV infection occurs in 0.2-1% of all births, and causes birth defects and developmental abnormalities, including sensorineural hearing loss and developmental delay. Several key studies have established the guinea pig as a tractable model for the study of congenital HCMV infection and have shown that polyclonal antibodies can be protective. In this study, we demonstrate that an anti-guinea pig CMV (GPCMV) glycoprotein H/glycoprotein L neutralizing monoclonal antibody protects against fetal infection and loss in the guinea pig. Furthermore, we have delineated the kinetics of GPCMV congenital infection, from maternal infection (salivary glands, seroconversion, placenta) to fetal infection (fetus and amniotic fluid). Our studies support the hypothesis that a neutralizing monoclonal antibody targeting an envelope GPCMV glycoprotein can protect the fetus from infection and may shed light on the therapeutic intervention of HCMV congenital infection in humans.http://europepmc.org/articles/PMC3983071?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marcy R Auerbach Donghong Yan Rajesh Vij Jo-Anne Hongo Gerald Nakamura Jean-Michel Vernes Y Gloria Meng Samantha Lein Pamela Chan Jed Ross Richard Carano Rong Deng Nicholas Lewin-Koh Min Xu Becket Feierbach |
spellingShingle |
Marcy R Auerbach Donghong Yan Rajesh Vij Jo-Anne Hongo Gerald Nakamura Jean-Michel Vernes Y Gloria Meng Samantha Lein Pamela Chan Jed Ross Richard Carano Rong Deng Nicholas Lewin-Koh Min Xu Becket Feierbach A neutralizing anti-gH/gL monoclonal antibody is protective in the guinea pig model of congenital CMV infection. PLoS Pathogens |
author_facet |
Marcy R Auerbach Donghong Yan Rajesh Vij Jo-Anne Hongo Gerald Nakamura Jean-Michel Vernes Y Gloria Meng Samantha Lein Pamela Chan Jed Ross Richard Carano Rong Deng Nicholas Lewin-Koh Min Xu Becket Feierbach |
author_sort |
Marcy R Auerbach |
title |
A neutralizing anti-gH/gL monoclonal antibody is protective in the guinea pig model of congenital CMV infection. |
title_short |
A neutralizing anti-gH/gL monoclonal antibody is protective in the guinea pig model of congenital CMV infection. |
title_full |
A neutralizing anti-gH/gL monoclonal antibody is protective in the guinea pig model of congenital CMV infection. |
title_fullStr |
A neutralizing anti-gH/gL monoclonal antibody is protective in the guinea pig model of congenital CMV infection. |
title_full_unstemmed |
A neutralizing anti-gH/gL monoclonal antibody is protective in the guinea pig model of congenital CMV infection. |
title_sort |
neutralizing anti-gh/gl monoclonal antibody is protective in the guinea pig model of congenital cmv infection. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Pathogens |
issn |
1553-7366 1553-7374 |
publishDate |
2014-04-01 |
description |
Human cytomegalovirus (HCMV) is the most common cause of congenital virus infection. Congenital HCMV infection occurs in 0.2-1% of all births, and causes birth defects and developmental abnormalities, including sensorineural hearing loss and developmental delay. Several key studies have established the guinea pig as a tractable model for the study of congenital HCMV infection and have shown that polyclonal antibodies can be protective. In this study, we demonstrate that an anti-guinea pig CMV (GPCMV) glycoprotein H/glycoprotein L neutralizing monoclonal antibody protects against fetal infection and loss in the guinea pig. Furthermore, we have delineated the kinetics of GPCMV congenital infection, from maternal infection (salivary glands, seroconversion, placenta) to fetal infection (fetus and amniotic fluid). Our studies support the hypothesis that a neutralizing monoclonal antibody targeting an envelope GPCMV glycoprotein can protect the fetus from infection and may shed light on the therapeutic intervention of HCMV congenital infection in humans. |
url |
http://europepmc.org/articles/PMC3983071?pdf=render |
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