Homology-independent multiallelic disruption via CRISPR/Cas9-based knock-in yields distinct functional outcomes in human cells

Homology-independent multiallelic disruption via CRISPR/Cas9-based knock-in yields distinct functional outcomes in human cells

Abstract Background Cultured human cells are pivotal models to study human gene functions, but introducing complete loss of function in diploid or aneuploid cells has been a challenge. The recently developed CRISPR/Cas9-mediated homology-independent knock-in approach permits targeted insertion of la...

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Bibliographic Details
Main Authors: Chenzi Zhang, Xiangjun He, Yvonne K. Kwok, Feng Wang, Junyi Xue, Hui Zhao, Kin Wah Suen, Chi Chiu Wang, Jianwei Ren, George G. Chen, Paul B. S. Lai, Jiangchao Li, Yin Xia, Andrew M. Chan, Wai-Yee Chan, Bo Feng
Format: Article
Language:English
Published: BMC 2018-12-01
Series:BMC Biology
Subjects:
Multiallelic gene disruption
Homology-independent knock-in
Loss-of-function
Hyperploid cells
UlK1
FAT10
Online Access:http://link.springer.com/article/10.1186/s12915-018-0616-2

Internet

http://link.springer.com/article/10.1186/s12915-018-0616-2

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