High-Efficiency Lentiviral Transduction of Human CD34+ Cells in High-Density Culture with Poloxamer and Prostaglandin E2

High-Efficiency Lentiviral Transduction of Human CD34+ Cells in High-Density Culture with Poloxamer and Prostaglandin E2

Hematopoietic stem cell (HSC) gene therapy is curative for various hereditary diseases; however, high-efficiency transduction in HSCs remains crucial to improve the prospects for hemoglobinopathies. We previously optimized lentiviral transduction in human CD34+ cells with serum-free medium containin...

Full description

Bibliographic Details
Main Authors: Naoya Uchida, Tina Nassehi, Claire M. Drysdale, Jackson Gamer, Morgan Yapundich, Selami Demirci, Juan J. Haro-Mora, Alexis Leonard, Matthew M. Hsieh, John F. Tisdale
Format: Article
Language:English
Published: Elsevier 2019-06-01
Series:Molecular Therapy: Methods & Clinical Development
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050119300087

Internet

http://www.sciencedirect.com/science/article/pii/S2329050119300087

Similar Items