High-Efficiency Lentiviral Transduction of Human CD34+ Cells in High-Density Culture with Poloxamer and Prostaglandin E2
Hematopoietic stem cell (HSC) gene therapy is curative for various hereditary diseases; however, high-efficiency transduction in HSCs remains crucial to improve the prospects for hemoglobinopathies. We previously optimized lentiviral transduction in human CD34+ cells with serum-free medium containin...
Main Authors: | Naoya Uchida, Tina Nassehi, Claire M. Drysdale, Jackson Gamer, Morgan Yapundich, Selami Demirci, Juan J. Haro-Mora, Alexis Leonard, Matthew M. Hsieh, John F. Tisdale |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2019-06-01
|
Series: | Molecular Therapy: Methods & Clinical Development |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2329050119300087 |
Similar Items
-
Low-Dose Busulfan Reduces Human CD34+ Cell Doses Required for Engraftment in c-kit Mutant Immunodeficient Mice
by: Alexis Leonard, et al.
Published: (2019-12-01) -
Cas9 protein delivery non-integrating lentiviral vectors for gene correction in sickle cell disease
by: Naoya Uchida, et al.
Published: (2021-06-01) -
Decitabine suspends human CD34+ cell differentiation and proliferation during lentiviral transduction.
by: Naoya Uchida, et al.
Published: (2014-01-01) -
βT87Q-Globin Gene Therapy Reduces Sickle Hemoglobin Production, Allowing for Ex Vivo Anti-sickling Activity in Human Erythroid Cells
by: Selami Demirci, et al.
Published: (2020-06-01) -
PGE2 and Poloxamer Synperonic F108 Enhance Transduction of Human HSPCs with a β-Globin Lentiviral Vector
by: Katelyn E. Masiuk, et al.
Published: (2019-06-01)