Evaluation of Established Methods for DNA Extraction and Primer Pairs Targeting 16S rRNA Gene for Bacterial Microbiota Profiling of Olive Xylem Sap

Evaluation of Established Methods for DNA Extraction and Primer Pairs Targeting 16S rRNA Gene for Bacterial Microbiota Profiling of Olive Xylem Sap

Next-generation sequencing has revolutionized our ability to investigate the microbiota composition of diverse and complex environments. However, a number of factors can affect the accuracy of microbial community assessment, such as the DNA extraction method, the hypervariable region of 16S rRNA gen...

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Bibliographic Details
Main Authors: Carmen Haro, Manuel Anguita-Maeso, Madis Metsis, Juan A. Navas-Cortés, Blanca B. Landa
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-03-01
Series:Frontiers in Plant Science
Subjects:
DNA extraction kits
NGS
16S rRNA gene
mock community
microbiome
xylem
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2021.640829/full

Internet

https://www.frontiersin.org/articles/10.3389/fpls.2021.640829/full

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