Evaluation of Established Methods for DNA Extraction and Primer Pairs Targeting 16S rRNA Gene for Bacterial Microbiota Profiling of Olive Xylem Sap
Next-generation sequencing has revolutionized our ability to investigate the microbiota composition of diverse and complex environments. However, a number of factors can affect the accuracy of microbial community assessment, such as the DNA extraction method, the hypervariable region of 16S rRNA gen...
Main Authors: | Carmen Haro, Manuel Anguita-Maeso, Madis Metsis, Juan A. Navas-Cortés, Blanca B. Landa |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2021-03-01
|
Series: | Frontiers in Plant Science |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.640829/full |
Similar Items
-
Culture-Dependent and Culture-Independent Characterization of the Olive Xylem Microbiota: Effect of Sap Extraction Methods
by: Manuel Anguita-Maeso, et al.
Published: (2020-01-01) -
Metabolomic, Ionomic and Microbial Characterization of Olive Xylem Sap Reveals Differences According to Plant Age and Genotype
by: Manuel Anguita-Maeso, et al.
Published: (2021-06-01) -
Verticillium dahliae Inoculation and in vitro Propagation Modify the Xylem Microbiome and Disease Reaction to Verticillium Wilt in a Wild Olive Genotype
by: Manuel Anguita-Maeso, et al.
Published: (2021-03-01) -
Metabolite Profile of Xylem Sap in Cotton Seedlings Is Changed by K Deficiency
by: Xin Zhang, et al.
Published: (2020-12-01) -
Detection of Oxytetracycline in Citrus Phloem and Xylem Saps Using Europium-Based Method
by: Faraj Hijaz, et al.
Published: (2021-08-01)