OPTIMAL PARAMETERS FOR PLASMID DNA PREPARATION USING THE ALKALINE LYSIS METHOD
The great variety of different parameters found in the literature when preparing plasmid DNA from Escherichia coli makes difficult to established the best method in terms of yield, reproducibility and speed. In this study several parameters were addressed, including temperature and incubation time...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
"Alexandru Ioan Cuza" University of Iași
2007-12-01
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Series: | Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara |
Online Access: | http://www.gbm.bio.uaic.ro/index.php/gbm/article/view/423 |