OPTIMAL PARAMETERS FOR PLASMID DNA PREPARATION USING THE ALKALINE LYSIS METHOD

The great variety of different parameters found in the literature when preparing plasmid DNA from Escherichia coli makes difficult to established the best method in terms of yield, reproducibility and speed. In this study several parameters were addressed, including temperature and incubation time...

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Bibliographic Details
Main Authors: Marius Mihasan, Alina Nita, Vlad Artenie
Format: Article
Language:English
Published: "Alexandru Ioan Cuza" University of Iași 2007-12-01
Series:Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara
Online Access:http://www.gbm.bio.uaic.ro/index.php/gbm/article/view/423