Application of uMelt SM algorithm in Real-time PCR melting curve analysis

One of the challenges in interpreting the results obtained in qPCR is to make sure the amplicons are specific and that the melting curve analysis is used to examine it.  Additional peaks in the melting curve is not always indicative of a problem, for this purpose, in this paper, a web-based tool cal...

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Bibliographic Details
Main Authors: Anahita Kharabi, Abbas Saedi
Format: Article
Language:fas
Published: Shahid Bahonar University of Kerman 2018-09-01
Series:مجله بیوتکنولوژی کشاورزی
Subjects:
Online Access:https://jab.uk.ac.ir/article_2150_d7eeb631ca4f4b97ac4e8d6a674196fa.pdf