Distinct Mutation Patterns Reveal Melanoma Subtypes and Influence Immunotherapy Response in Advanced Melanoma Patients
The detection of somatic driver mutations by next-generation sequencing (NGS) is becoming increasingly important in the care of advanced melanoma patients. In our study, we evaluated the NGS results of 82 melanoma patients from clinical routine in 2017. Besides determining the tumor mutational burde...
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doaj-bb977030db0848e0891f97bb7971a10e2020-11-25T03:42:12ZengMDPI AGCancers2072-66942020-08-01122359235910.3390/cancers12092359Distinct Mutation Patterns Reveal Melanoma Subtypes and Influence Immunotherapy Response in Advanced Melanoma PatientsFranz J. Hilke0Tobias Sinnberg1Axel Gschwind2Heike Niessner3German Demidov4Teresa Amaral5Stephan Ossowski6Irina Bonzheim7Martin Röcken8Olaf Riess9Claus Garbe10Christopher Schroeder11Andrea Forschner12Institute of Medical Genetics and Applied Genomics, University Hospital Tübingen, 72076 Tübingen, GermanyImage-Guided and Functionally Instructed Tumor Therapies (iFIT) Cluster of Excellence (EXC 2180), University of Tübingen, 72076 Tübingen, GermanyInstitute of Medical Genetics and Applied Genomics, University Hospital Tübingen, 72076 Tübingen, GermanyDepartment of Dermatology, University Hospital Tübingen, 72076 Tübingen, GermanyInstitute of Medical Genetics and Applied Genomics, University Hospital Tübingen, 72076 Tübingen, GermanyDepartment of Dermatology, University Hospital Tübingen, 72076 Tübingen, GermanyInstitute of Medical Genetics and Applied Genomics, University Hospital Tübingen, 72076 Tübingen, GermanyInstitute of Pathology and Neuropathology, University Hospital Tübingen, 72076 Tübingen, GermanyDepartment of Dermatology, University Hospital Tübingen, 72076 Tübingen, GermanyInstitute of Medical Genetics and Applied Genomics, University Hospital Tübingen, 72076 Tübingen, GermanyDepartment of Dermatology, University Hospital Tübingen, 72076 Tübingen, GermanyInstitute of Medical Genetics and Applied Genomics, University Hospital Tübingen, 72076 Tübingen, GermanyDepartment of Dermatology, University Hospital Tübingen, 72076 Tübingen, GermanyThe detection of somatic driver mutations by next-generation sequencing (NGS) is becoming increasingly important in the care of advanced melanoma patients. In our study, we evaluated the NGS results of 82 melanoma patients from clinical routine in 2017. Besides determining the tumor mutational burden (TMB) and annotation of all genetic driver alterations, we investigated their potential as a predictor for resistance to immune checkpoint inhibitors (ICI) and as a distinguishing feature between melanoma subtypes. Melanomas of unknown primary had a similar mutation pattern and TMB to cutaneous melanoma, which hints at its cutaneous origin. Besides the typical hotspot mutation in <i>BRAF</i> and <i>NRAS</i>, we frequently observed <i>CDKN2A</i> deletions. Acral and mucosal melanomas were dominated by CNV alterations affecting <i>PDGFRA</i>, <i>KIT</i>, <i>CDK4</i>, <i>RICTOR</i>, <i>CCND2</i> and <i>CHEK2</i>. Uveal melanoma often had somatic SNVs in <i>GNA11/Q</i> and amplification of <i>MYC</i> in all cases. A significantly higher incidence of <i>BRAF</i> V600 mutations and <i>EGFR</i> amplifications, <i>PTEN</i> and <i>TP53</i> deletions was found in patients with disease progression while on ICI. Thus, NGS might help to characterize melanoma subtypes more precisely and to identify possible resistance mechanisms to ICI therapy. Nevertheless, NGS based studies, including larger cohorts, are needed to support potential genetic ICI resistance mechanisms.https://www.mdpi.com/2072-6694/12/9/2359Genome of advanced melanomaacralmucosaluvealmelanoma of unknown origintumor mutation burden |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Franz J. Hilke Tobias Sinnberg Axel Gschwind Heike Niessner German Demidov Teresa Amaral Stephan Ossowski Irina Bonzheim Martin Röcken Olaf Riess Claus Garbe Christopher Schroeder Andrea Forschner |
spellingShingle |
Franz J. Hilke Tobias Sinnberg Axel Gschwind Heike Niessner German Demidov Teresa Amaral Stephan Ossowski Irina Bonzheim Martin Röcken Olaf Riess Claus Garbe Christopher Schroeder Andrea Forschner Distinct Mutation Patterns Reveal Melanoma Subtypes and Influence Immunotherapy Response in Advanced Melanoma Patients Cancers Genome of advanced melanoma acral mucosal uveal melanoma of unknown origin tumor mutation burden |
author_facet |
Franz J. Hilke Tobias Sinnberg Axel Gschwind Heike Niessner German Demidov Teresa Amaral Stephan Ossowski Irina Bonzheim Martin Röcken Olaf Riess Claus Garbe Christopher Schroeder Andrea Forschner |
author_sort |
Franz J. Hilke |
title |
Distinct Mutation Patterns Reveal Melanoma Subtypes and Influence Immunotherapy Response in Advanced Melanoma Patients |
title_short |
Distinct Mutation Patterns Reveal Melanoma Subtypes and Influence Immunotherapy Response in Advanced Melanoma Patients |
title_full |
Distinct Mutation Patterns Reveal Melanoma Subtypes and Influence Immunotherapy Response in Advanced Melanoma Patients |
title_fullStr |
Distinct Mutation Patterns Reveal Melanoma Subtypes and Influence Immunotherapy Response in Advanced Melanoma Patients |
title_full_unstemmed |
Distinct Mutation Patterns Reveal Melanoma Subtypes and Influence Immunotherapy Response in Advanced Melanoma Patients |
title_sort |
distinct mutation patterns reveal melanoma subtypes and influence immunotherapy response in advanced melanoma patients |
publisher |
MDPI AG |
series |
Cancers |
issn |
2072-6694 |
publishDate |
2020-08-01 |
description |
The detection of somatic driver mutations by next-generation sequencing (NGS) is becoming increasingly important in the care of advanced melanoma patients. In our study, we evaluated the NGS results of 82 melanoma patients from clinical routine in 2017. Besides determining the tumor mutational burden (TMB) and annotation of all genetic driver alterations, we investigated their potential as a predictor for resistance to immune checkpoint inhibitors (ICI) and as a distinguishing feature between melanoma subtypes. Melanomas of unknown primary had a similar mutation pattern and TMB to cutaneous melanoma, which hints at its cutaneous origin. Besides the typical hotspot mutation in <i>BRAF</i> and <i>NRAS</i>, we frequently observed <i>CDKN2A</i> deletions. Acral and mucosal melanomas were dominated by CNV alterations affecting <i>PDGFRA</i>, <i>KIT</i>, <i>CDK4</i>, <i>RICTOR</i>, <i>CCND2</i> and <i>CHEK2</i>. Uveal melanoma often had somatic SNVs in <i>GNA11/Q</i> and amplification of <i>MYC</i> in all cases. A significantly higher incidence of <i>BRAF</i> V600 mutations and <i>EGFR</i> amplifications, <i>PTEN</i> and <i>TP53</i> deletions was found in patients with disease progression while on ICI. Thus, NGS might help to characterize melanoma subtypes more precisely and to identify possible resistance mechanisms to ICI therapy. Nevertheless, NGS based studies, including larger cohorts, are needed to support potential genetic ICI resistance mechanisms. |
topic |
Genome of advanced melanoma acral mucosal uveal melanoma of unknown origin tumor mutation burden |
url |
https://www.mdpi.com/2072-6694/12/9/2359 |
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