Impact of Larger Sputum Volume on Xpert® MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis

Impact of Larger Sputum Volume on Xpert® MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis

As a strategy to improve the sensitivity of nucleic acid-based testing in acid-fast bacilli (AFB) negative samples, larger volumes of sputum (5–10 mL) were tested with Xpert® MTB/RIF from 176 individuals with smear-negative sputum undergoing tuberculosis evaluation. Despite larger volumes, this stra...

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Main Authors: Sharlaa Badal-Faesen, Cynthia Firnhaber, Michelle A. Kendall, Xingye Wu, Beatriz Grinsztejn, Rodrigo Otavio da Silva Escada, Michel Fernandez, Evelyn Hogg, Ian Sanne, Pamela Johnson, David Alland, Gerald H. Mazurek, Debra A. Benator, Anne F. Luetkemeyer, the AIDS Clinical Trials Group A5295, Tuberculosis Trials Consortium Study 34 Teams
Format: Article
Language:English
Published: MDPI AG 2017-08-01
Series:Journal of Clinical Medicine
Subjects:
larger volume sputum
Xpert MTB/RIF
smear-negative suspects
Online Access:https://www.mdpi.com/2077-0383/6/8/78
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spelling doaj-bb97ab2977b94c54b5bc0df917778fad2020-11-24T22:04:13ZengMDPI AGJournal of Clinical Medicine2077-03832017-08-01687810.3390/jcm6080078jcm6080078Impact of Larger Sputum Volume on Xpert® MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected TuberculosisSharlaa Badal-Faesen0Cynthia Firnhaber1Michelle A. Kendall2Xingye Wu3Beatriz Grinsztejn4Rodrigo Otavio da Silva Escada5Michel Fernandez6Evelyn Hogg7Ian Sanne8Pamela Johnson9David Alland10Gerald H. Mazurek11Debra A. Benator12Anne F. Luetkemeyer13the AIDS Clinical Trials Group A529514Tuberculosis Trials Consortium Study 34 Teams15Clinical HIV Research Unit, Department of Internal Medicine, School of Clinical Medicine, Faculty of Health Sciences, University of Witwatersrand, Johannesburg 2092, South AfricaClinical HIV Research Unit, Department of Internal Medicine, School of Clinical Medicine, Faculty of Health Sciences, University of Witwatersrand, Johannesburg 2092, South AfricaCenter for Biostatistics in AIDS Research, Harvard T.H. Chan School of Public Health Boston, Boston, MA 02115, USACenter for Biostatistics in AIDS Research, Harvard T.H. Chan School of Public Health Boston, Boston, MA 02115, USAInstituto Nacional de Infectologia Evandro Chagas-INI-Fiocruz, Rio de Janeiro 21.045-900, BrazilInstituto Nacional de Infectologia Evandro Chagas-INI-Fiocruz, Rio de Janeiro 21.045-900, BrazilPulmonary and Critical Care Department, Baylor Scott and White Hospital, Temple, TX 76508, USASocial & Scientific Systems, Inc., Silver Spring, MD 20910, USAClinical HIV Research Unit, Department of Internal Medicine, School of Clinical Medicine, Faculty of Health Sciences, University of Witwatersrand, Johannesburg 2092, South AfricaCepheid Inc., Sunnyvale, CA 94089, USADivision of Infectious Disease, Rutgers-New Jersey Medical School, 185 South Orange Avenue, Newark, NJ 07103, USADivision of Tuberculosis Elimination, Centers for Disease Control and Prevention, Atlanta, GA 30333, USAThe Veterans Affairs Medical Center, The George Washington University, Washington, DC 20422, USADivision of HIV, Infectious Diseases and Global Medicine, Zuckerberg San Francisco General, University of California, San Francisco, CA 94110, USAClinical HIV Research Unit, Department of Internal Medicine, School of Clinical Medicine, Faculty of Health Sciences, University of Witwatersrand, Johannesburg 2092, South AfricaClinical HIV Research Unit, Department of Internal Medicine, School of Clinical Medicine, Faculty of Health Sciences, University of Witwatersrand, Johannesburg 2092, South AfricaAs a strategy to improve the sensitivity of nucleic acid-based testing in acid-fast bacilli (AFB) negative samples, larger volumes of sputum (5–10 mL) were tested with Xpert® MTB/RIF from 176 individuals with smear-negative sputum undergoing tuberculosis evaluation. Despite larger volumes, this strategy had a suboptimal sensitivity of 50% (4/8).https://www.mdpi.com/2077-0383/6/8/78larger volume sputumXpert MTB/RIFsmear-negative suspects
collection DOAJ
language English
format Article
sources DOAJ
author Sharlaa Badal-Faesen
Cynthia Firnhaber
Michelle A. Kendall
Xingye Wu
Beatriz Grinsztejn
Rodrigo Otavio da Silva Escada
Michel Fernandez
Evelyn Hogg
Ian Sanne
Pamela Johnson
David Alland
Gerald H. Mazurek
Debra A. Benator
Anne F. Luetkemeyer
the AIDS Clinical Trials Group A5295
Tuberculosis Trials Consortium Study 34 Teams
spellingShingle Sharlaa Badal-Faesen
Cynthia Firnhaber
Michelle A. Kendall
Xingye Wu
Beatriz Grinsztejn
Rodrigo Otavio da Silva Escada
Michel Fernandez
Evelyn Hogg
Ian Sanne
Pamela Johnson
David Alland
Gerald H. Mazurek
Debra A. Benator
Anne F. Luetkemeyer
the AIDS Clinical Trials Group A5295
Tuberculosis Trials Consortium Study 34 Teams
Impact of Larger Sputum Volume on Xpert® MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis
Journal of Clinical Medicine
larger volume sputum
Xpert MTB/RIF
smear-negative suspects
author_facet Sharlaa Badal-Faesen
Cynthia Firnhaber
Michelle A. Kendall
Xingye Wu
Beatriz Grinsztejn
Rodrigo Otavio da Silva Escada
Michel Fernandez
Evelyn Hogg
Ian Sanne
Pamela Johnson
David Alland
Gerald H. Mazurek
Debra A. Benator
Anne F. Luetkemeyer
the AIDS Clinical Trials Group A5295
Tuberculosis Trials Consortium Study 34 Teams
author_sort Sharlaa Badal-Faesen
title Impact of Larger Sputum Volume on Xpert® MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis
title_short Impact of Larger Sputum Volume on Xpert® MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis
title_full Impact of Larger Sputum Volume on Xpert® MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis
title_fullStr Impact of Larger Sputum Volume on Xpert® MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis
title_full_unstemmed Impact of Larger Sputum Volume on Xpert® MTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis
title_sort impact of larger sputum volume on xpert® mtb/rif assay detection of mycobacterium tuberculosis in smear-negative individuals with suspected tuberculosis
publisher MDPI AG
series Journal of Clinical Medicine
issn 2077-0383
publishDate 2017-08-01
description As a strategy to improve the sensitivity of nucleic acid-based testing in acid-fast bacilli (AFB) negative samples, larger volumes of sputum (5–10 mL) were tested with Xpert® MTB/RIF from 176 individuals with smear-negative sputum undergoing tuberculosis evaluation. Despite larger volumes, this strategy had a suboptimal sensitivity of 50% (4/8).
topic larger volume sputum
Xpert MTB/RIF
smear-negative suspects
url https://www.mdpi.com/2077-0383/6/8/78
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