Patch-clamp recordings and calcium imaging followed by single-cell PCR reveal the developmental profile of 13 genes in iPSC-derived human neurons

Patch-clamp recordings and calcium imaging followed by single-cell PCR reveal the developmental profile of 13 genes in iPSC-derived human neurons

Molecular genetic studies are typically performed on homogenized biological samples, resulting in contamination from non-neuronal cells. To improve expression profiling of neurons we combined patch recordings with single-cell PCR. Two iPSC lines (healthy subject and 22q11.2 deletion) were differenti...

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Bibliographic Details
Main Authors: Glenn S. Belinsky, Matthew T. Rich, Carissa L. Sirois, Shaina M. Short, Erika Pedrosa, Herbert M. Lachman, Srdjan D. Antic
Format: Article
Language:English
Published: Elsevier 2014-01-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506113001402

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http://www.sciencedirect.com/science/article/pii/S1873506113001402

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