Reliable CRISPR/Cas9 Genome Engineering in Caenorhabditis elegans Using a Single Efficient sgRNA and an Easily Recognizable Phenotype

Reliable CRISPR/Cas9 Genome Engineering in Caenorhabditis elegans Using a Single Efficient sgRNA and an Easily Recognizable Phenotype

CRISPR/Cas9 genome engineering strategies allow the directed modification of the Caenorhabditis elegans genome to introduce point mutations, generate knock-out mutants, and insert coding sequences for epitope or fluorescent tags. Three practical aspects, however, complicate such experiments. First,...

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Bibliographic Details
Main Authors: Sonia El Mouridi, Claire Lecroisey, Philippe Tardy, Marine Mercier, Alice Leclercq-Blondel, Nora Zariohi, Thomas Boulin
Format: Article
Language:English
Published: Oxford University Press 2017-05-01
Series:G3: Genes, Genomes, Genetics
Subjects:
CRISPR/Cas9 genome engineering
Caenorhabditis elegans
mScarlet
dpy-10
coconversion
Online Access:http://g3journal.org/lookup/doi/10.1534/g3.117.040824

Internet

http://g3journal.org/lookup/doi/10.1534/g3.117.040824

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