CRISPR/Cas9‐enhanced ssDNA recombineering for Pseudomonas putida

Summary Implementation of single‐stranded DNA (ssDNA) recombineering in Pseudomonas putida has widened the range of genetic manipulations applicable to this biotechnologically relevant bacterium. Yet, the relatively low efficiency of the technology hampers identification of mutated clones lacking co...

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Bibliographic Details
Main Authors: Tomás Aparicio, Víctor deLorenzo, Esteban Martínez‐García
Format: Article
Language:English
Published: Wiley 2019-09-01
Series:Microbial Biotechnology
Online Access:https://doi.org/10.1111/1751-7915.13453