Easy and quick (EQ) sperm freezing method for urgent preservation of mouse strains

Abstract Cryopreservation of mouse spermatozoa is widely used for the efficient preservation and safe transport of valuable mouse strains. However, the current cryopreservation method requires special containers (plastic straws), undefined chemicals (e.g., skim milk), liquid nitrogen, and expertise...

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Bibliographic Details
Main Authors: Keiji Mochida, Ayumi Hasegawa, Daiki Shikata, Nobuhiko Itami, Masashi Hada, Naomi Watanabe, Toshiko Tomishima, Atsuo Ogura
Format: Article
Language:English
Published: Nature Publishing Group 2021-07-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-93604-y