Screening for functional IRESes using α-complementation system of β-galactosidase in Pichia pastoris

Screening for functional IRESes using α-complementation system of β-galactosidase in Pichia pastoris

Abstract Background Pichia pastoris is becoming a promising chassis cell for metabolic engineering and synthetic biology after its whole genome and transcriptome sequenced. However, the current systems for multigene co-expression in P. pastoris are not efficient. The internal ribosome entry site (IR...

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Bibliographic Details
Main Authors: Yide Huang, Yafei Zhang, Suhuan Li, Ting Lin, Jingwen Wu, Yao Lin
Format: Article
Language:English
Published: BMC 2019-12-01
Series:Biotechnology for Biofuels
Subjects:
Pichia pastoris
IRES
β-Galactosidase
α-Complementation
Multigene co-expression
Online Access:https://doi.org/10.1186/s13068-019-1640-3

Internet

https://doi.org/10.1186/s13068-019-1640-3

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