Influence of ?S-globin haplotypes and hydroxyurea on tumor necrosis factor-alpha levels in sickle cell anemia
Background: Sickle cell anemia is a chronic inflammatory disease characterized by an increased production of proinflammatory cytokines including tumor necrosis factor-alpha. Hydroxyurea, by decreasing the polymerization of hemoglobin, reduces inflammatory states. The effect of the genetic polymorphi...
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doaj-bc79b1365c8f45f495bd1ae31b70bb592020-11-24T21:29:57ZengElsevierRevista Brasileira de Hematologia e Hemoterapia1806-08702014-04-0136212112510.5581/1516-8484.20140028S1516-84842014000200121Influence of ?S-globin haplotypes and hydroxyurea on tumor necrosis factor-alpha levels in sickle cell anemiaMarília Rocha LaurentinoPedro Aurio Maia FilhoMaritza Cavalcante BarbosaIzabel Cristina Justino BandeiraLilianne Brito da Silva RochaRomelia Pinheiro GonçalvesBackground: Sickle cell anemia is a chronic inflammatory disease characterized by an increased production of proinflammatory cytokines including tumor necrosis factor-alpha. Hydroxyurea, by decreasing the polymerization of hemoglobin, reduces inflammatory states. The effect of the genetic polymorphisms of sickle cell patients on tumor necrosis factor-alpha levels remains unknown. Objective: The aim of this study was to investigate the association of tumor necrosis factor-alpha levels with β-globin haplotypes and the use of hydroxyurea. Methods: A cross-sectional study was performed of 67 patients with sickle cell anemia diagnosed at steady-state in a referral hospital in Fortaleza, Ceará, Brazil. A group of 26 healthy individuals was used as control. βS-haplotype analysis was performed by restriction fragment length polymorphism-polymerase chain reaction. The tumor necrosis factor-alpha levels were measured by the enzyme-linked immunosorbent assay test. Laboratory data (complete blood count and fetal hemoglobin) and information regarding the use of hydroxyurea were obtained from medical records. Statistical analysis was performed using R software with the Kruskal-Wallis and Mann-Whitney tests. Statistical significance was established for p-values < 0.05 for all analyses. Results: The mean age of the participants was 35.48 years. Patients with sickle cell anemia had significantly higher tumor necrosis factor-alpha levels than controls (p-values < 0.0001). Tumor necrosis factor-alpha levels were lower in sickle cell anemia patients who were receiving hydroxyurea treatment than those who were not (p-value = 0.1249). Sickle cell anemia patients with Bantu/n genotype had significantly higher levels than patients with the Bantu/Benin genotype (p-value = 0.0021). Conclusion: In summary, βS-globin haplotypes, but not hydroxyurea therapy, have a role in modulating tumor necrosis factor-alpha levels in sickle cell anemia adults at steady-state. Many previous studies have investigated prognosis and inflammatory states in sickle cell anemia patients, but the discovery that tumor necrosis factor-alpha levels vary according to the genetic polymorphism of the patient is a new finding.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842014000200121&lng=en&tlng=enAnemia, sickle cellInflammationTumor necrosis factor-alphaHaplotypesHydroxyurea |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marília Rocha Laurentino Pedro Aurio Maia Filho Maritza Cavalcante Barbosa Izabel Cristina Justino Bandeira Lilianne Brito da Silva Rocha Romelia Pinheiro Gonçalves |
spellingShingle |
Marília Rocha Laurentino Pedro Aurio Maia Filho Maritza Cavalcante Barbosa Izabel Cristina Justino Bandeira Lilianne Brito da Silva Rocha Romelia Pinheiro Gonçalves Influence of ?S-globin haplotypes and hydroxyurea on tumor necrosis factor-alpha levels in sickle cell anemia Revista Brasileira de Hematologia e Hemoterapia Anemia, sickle cell Inflammation Tumor necrosis factor-alpha Haplotypes Hydroxyurea |
author_facet |
Marília Rocha Laurentino Pedro Aurio Maia Filho Maritza Cavalcante Barbosa Izabel Cristina Justino Bandeira Lilianne Brito da Silva Rocha Romelia Pinheiro Gonçalves |
author_sort |
Marília Rocha Laurentino |
title |
Influence of ?S-globin haplotypes and hydroxyurea on tumor necrosis factor-alpha levels in sickle cell anemia |
title_short |
Influence of ?S-globin haplotypes and hydroxyurea on tumor necrosis factor-alpha levels in sickle cell anemia |
title_full |
Influence of ?S-globin haplotypes and hydroxyurea on tumor necrosis factor-alpha levels in sickle cell anemia |
title_fullStr |
Influence of ?S-globin haplotypes and hydroxyurea on tumor necrosis factor-alpha levels in sickle cell anemia |
title_full_unstemmed |
Influence of ?S-globin haplotypes and hydroxyurea on tumor necrosis factor-alpha levels in sickle cell anemia |
title_sort |
influence of ?s-globin haplotypes and hydroxyurea on tumor necrosis factor-alpha levels in sickle cell anemia |
publisher |
Elsevier |
series |
Revista Brasileira de Hematologia e Hemoterapia |
issn |
1806-0870 |
publishDate |
2014-04-01 |
description |
Background: Sickle cell anemia is a chronic inflammatory disease characterized by an increased production of proinflammatory cytokines including tumor necrosis factor-alpha. Hydroxyurea, by decreasing the polymerization of hemoglobin, reduces inflammatory states. The effect of the genetic polymorphisms of sickle cell patients on tumor necrosis factor-alpha levels remains unknown. Objective: The aim of this study was to investigate the association of tumor necrosis factor-alpha levels with β-globin haplotypes and the use of hydroxyurea. Methods: A cross-sectional study was performed of 67 patients with sickle cell anemia diagnosed at steady-state in a referral hospital in Fortaleza, Ceará, Brazil. A group of 26 healthy individuals was used as control. βS-haplotype analysis was performed by restriction fragment length polymorphism-polymerase chain reaction. The tumor necrosis factor-alpha levels were measured by the enzyme-linked immunosorbent assay test. Laboratory data (complete blood count and fetal hemoglobin) and information regarding the use of hydroxyurea were obtained from medical records. Statistical analysis was performed using R software with the Kruskal-Wallis and Mann-Whitney tests. Statistical significance was established for p-values < 0.05 for all analyses. Results: The mean age of the participants was 35.48 years. Patients with sickle cell anemia had significantly higher tumor necrosis factor-alpha levels than controls (p-values < 0.0001). Tumor necrosis factor-alpha levels were lower in sickle cell anemia patients who were receiving hydroxyurea treatment than those who were not (p-value = 0.1249). Sickle cell anemia patients with Bantu/n genotype had significantly higher levels than patients with the Bantu/Benin genotype (p-value = 0.0021). Conclusion: In summary, βS-globin haplotypes, but not hydroxyurea therapy, have a role in modulating tumor necrosis factor-alpha levels in sickle cell anemia adults at steady-state. Many previous studies have investigated prognosis and inflammatory states in sickle cell anemia patients, but the discovery that tumor necrosis factor-alpha levels vary according to the genetic polymorphism of the patient is a new finding. |
topic |
Anemia, sickle cell Inflammation Tumor necrosis factor-alpha Haplotypes Hydroxyurea |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842014000200121&lng=en&tlng=en |
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