The X-ray repair cross complementing protein 1 (XRCC1) rs25487 polymorphism and susceptibility to cirrhosis in Brazilian patients with chronic viral hepatitis
Introduction. The progression of hepatic disease in chronic viral hepatitis is accompanied by an increased production of reactive oxygen species (ROS), as well as an accumulation of oxidative DNA damage, which is primarily repaired through base excision repair. XRCC1 (X-ray repair cross complementin...
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doaj-bcc250321cba45998f82676a6d76daaf2021-06-09T05:54:06ZengElsevierAnnals of Hepatology1665-26812013-09-01125733739The X-ray repair cross complementing protein 1 (XRCC1) rs25487 polymorphism and susceptibility to cirrhosis in Brazilian patients with chronic viral hepatitisSamantha Therezinha Almeida Pereira Leite0Nathália Marques-Guimarães1Júlio César Silva-Oliveira2Francisco José Dutra-Souto3Raquel Alves-dos-Santos4Carmen Lucia Bassi-Branco5Pós-graduação em Ciencias da Saúde, Faculdade de Medicina (FM/UFMT), Cuiabá, BrazilFM/UFMT, Cuiabá, BrazilFM/UFMT, Cuiabá, BrazilDepartamento de Clínica Médica, FM/UFMT, Cuiabá, BrazilPós-graduação em Ciencias, Universidade de Franca (UNIFRAN), Franca, BrazilDepartamento de Ciências Básicas em Saúde, FM/UFMT, Cuiabá, Brazil; Correspondence and reprint request:Introduction. The progression of hepatic disease in chronic viral hepatitis is accompanied by an increased production of reactive oxygen species (ROS), as well as an accumulation of oxidative DNA damage, which is primarily repaired through base excision repair. XRCC1 (X-ray repair cross complementing protein 1) is one of the most important proteins involved in this repair pathway. The present study was carried out to verify the possible association of the XRCC1 rs25487 polymorphism with cirrhosis in patients from Central-West Brazil.Material and methods. A total of 227 individuals with viral hepatitis, 53 cirrhotic and 174 non-cirrhotic, were genotyped for the XRCC1 rs25487 polymorphism using PCR-RFLP. Results: There were significantly higher frequencies of both the Arg/Gln genotype and of individuals with at least one Gln allele (Arg/ Gln+Gln/Gln) among cirrhotic patients (56.6% and 69.8%) compared with non-cirrhotic patients (25.8% and 37.9%). Both conditions were significantly associated with cirrhosis, independent of age, sex, alcohol intake or tobacco use (adjusted OR = 3.5, CI = 1.7–7.4, p = 0.001 and adjusted OR = 3.1, CI = 1.5–6.3, p = 0.002, respectively). Similar results were obtained for a group of HCV-infected patients but not for HBV-in-fected patients. Conclusions. The XRCC1 rs25487 polymorphism may influence the development of cirrhosis in viral hepatitis patients, and additional investigation will be necessary.http://www.sciencedirect.com/science/article/pii/S1665268119313146DNA repairChronic hepatitis BChronic hepatitis CLiver cirrhosisSingle nucleotide polymorphism |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Samantha Therezinha Almeida Pereira Leite Nathália Marques-Guimarães Júlio César Silva-Oliveira Francisco José Dutra-Souto Raquel Alves-dos-Santos Carmen Lucia Bassi-Branco |
spellingShingle |
Samantha Therezinha Almeida Pereira Leite Nathália Marques-Guimarães Júlio César Silva-Oliveira Francisco José Dutra-Souto Raquel Alves-dos-Santos Carmen Lucia Bassi-Branco The X-ray repair cross complementing protein 1 (XRCC1) rs25487 polymorphism and susceptibility to cirrhosis in Brazilian patients with chronic viral hepatitis Annals of Hepatology DNA repair Chronic hepatitis B Chronic hepatitis C Liver cirrhosis Single nucleotide polymorphism |
author_facet |
Samantha Therezinha Almeida Pereira Leite Nathália Marques-Guimarães Júlio César Silva-Oliveira Francisco José Dutra-Souto Raquel Alves-dos-Santos Carmen Lucia Bassi-Branco |
author_sort |
Samantha Therezinha Almeida Pereira Leite |
title |
The X-ray repair cross complementing protein 1 (XRCC1) rs25487 polymorphism and susceptibility to cirrhosis in Brazilian patients with chronic viral hepatitis |
title_short |
The X-ray repair cross complementing protein 1 (XRCC1) rs25487 polymorphism and susceptibility to cirrhosis in Brazilian patients with chronic viral hepatitis |
title_full |
The X-ray repair cross complementing protein 1 (XRCC1) rs25487 polymorphism and susceptibility to cirrhosis in Brazilian patients with chronic viral hepatitis |
title_fullStr |
The X-ray repair cross complementing protein 1 (XRCC1) rs25487 polymorphism and susceptibility to cirrhosis in Brazilian patients with chronic viral hepatitis |
title_full_unstemmed |
The X-ray repair cross complementing protein 1 (XRCC1) rs25487 polymorphism and susceptibility to cirrhosis in Brazilian patients with chronic viral hepatitis |
title_sort |
x-ray repair cross complementing protein 1 (xrcc1) rs25487 polymorphism and susceptibility to cirrhosis in brazilian patients with chronic viral hepatitis |
publisher |
Elsevier |
series |
Annals of Hepatology |
issn |
1665-2681 |
publishDate |
2013-09-01 |
description |
Introduction. The progression of hepatic disease in chronic viral hepatitis is accompanied by an increased production of reactive oxygen species (ROS), as well as an accumulation of oxidative DNA damage, which is primarily repaired through base excision repair. XRCC1 (X-ray repair cross complementing protein 1) is one of the most important proteins involved in this repair pathway. The present study was carried out to verify the possible association of the XRCC1 rs25487 polymorphism with cirrhosis in patients from Central-West Brazil.Material and methods. A total of 227 individuals with viral hepatitis, 53 cirrhotic and 174 non-cirrhotic, were genotyped for the XRCC1 rs25487 polymorphism using PCR-RFLP. Results: There were significantly higher frequencies of both the Arg/Gln genotype and of individuals with at least one Gln allele (Arg/ Gln+Gln/Gln) among cirrhotic patients (56.6% and 69.8%) compared with non-cirrhotic patients (25.8% and 37.9%). Both conditions were significantly associated with cirrhosis, independent of age, sex, alcohol intake or tobacco use (adjusted OR = 3.5, CI = 1.7–7.4, p = 0.001 and adjusted OR = 3.1, CI = 1.5–6.3, p = 0.002, respectively). Similar results were obtained for a group of HCV-infected patients but not for HBV-in-fected patients. Conclusions. The XRCC1 rs25487 polymorphism may influence the development of cirrhosis in viral hepatitis patients, and additional investigation will be necessary. |
topic |
DNA repair Chronic hepatitis B Chronic hepatitis C Liver cirrhosis Single nucleotide polymorphism |
url |
http://www.sciencedirect.com/science/article/pii/S1665268119313146 |
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