A systems biology approach to explore the impact of maple tree dormancy release on sap variation and maple syrup quality
Abstract Maple sap is a complex nutrient matrix collected during spring to produce maple syrup. The characteristics of sap change over the production period and its composition directly impacts syrup quality. This variability could in part be attributed to changes in tree metabolism following dorman...
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doaj-bd76bebb0acf464c98f2c5ac5856a37f2020-12-08T05:31:35ZengNature Publishing GroupScientific Reports2045-23222018-10-018111310.1038/s41598-018-32940-yA systems biology approach to explore the impact of maple tree dormancy release on sap variation and maple syrup qualityGuillaume Quang N’guyen0Nathalie Martin1Mani Jain2Luc Lagacé3Christian R. Landry4Marie Filteau5Département des Sciences des aliments, Institut sur la nutrition et les aliments fonctionnels (INAF), Université LavalCentre de recherche, de développement et de transfert technologique acéricole Inc., Saint-Norbert-d’ArthabaskaDépartement de Biologie, Département de Biochimie, Microbiologie et Bio-informatique, PROTEO, Centre de recherche en données massives and Institut de Biologie Intégrative et des Systèmes (IBIS), Université LavalCentre de recherche, de développement et de transfert technologique acéricole Inc., Saint-Norbert-d’ArthabaskaDépartement de Biologie, Département de Biochimie, Microbiologie et Bio-informatique, PROTEO, Centre de recherche en données massives and Institut de Biologie Intégrative et des Systèmes (IBIS), Université LavalDépartement des Sciences des aliments, Institut sur la nutrition et les aliments fonctionnels (INAF), Université LavalAbstract Maple sap is a complex nutrient matrix collected during spring to produce maple syrup. The characteristics of sap change over the production period and its composition directly impacts syrup quality. This variability could in part be attributed to changes in tree metabolism following dormancy release, but little is known about these changes in deciduous trees. Therefore, understanding the variation in sap composition associated with dormancy release could help pinpoint the causes of some defects in maple syrup. In particular, a defect known as “buddy”, is an increasing concern for the industry. This off-flavor appears around the time of bud break, hence its name. To investigate sap variation related to bud break and the buddy defect, we monitored sap variation with respect to a dormancy release index (Sbb) and syrup quality. First, we looked at variation in amino acid content during this period. We observed a shift in amino acid relative proportions associated with dormancy release and found that most of them increase rapidly near the point of bud break, correlating with changes in syrup quality. Second, we identified biological processes that respond to variation in maple sap by performing a competition assay using the barcoded Saccharomyces cerevisiae prototroph deletion collection. This untargeted approach revealed that the organic sulfur content may be responsible for the development of the buddy off-flavor, and that dormancy release is necessary for the appearance of the defect, but other factors such as microbial activity may also be contributing.https://doi.org/10.1038/s41598-018-32940-yDormancy ReleaseSyrup QualityMaple TreesOrganic Sulfur ContentYeast Deletion Collection |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Guillaume Quang N’guyen Nathalie Martin Mani Jain Luc Lagacé Christian R. Landry Marie Filteau |
spellingShingle |
Guillaume Quang N’guyen Nathalie Martin Mani Jain Luc Lagacé Christian R. Landry Marie Filteau A systems biology approach to explore the impact of maple tree dormancy release on sap variation and maple syrup quality Scientific Reports Dormancy Release Syrup Quality Maple Trees Organic Sulfur Content Yeast Deletion Collection |
author_facet |
Guillaume Quang N’guyen Nathalie Martin Mani Jain Luc Lagacé Christian R. Landry Marie Filteau |
author_sort |
Guillaume Quang N’guyen |
title |
A systems biology approach to explore the impact of maple tree dormancy release on sap variation and maple syrup quality |
title_short |
A systems biology approach to explore the impact of maple tree dormancy release on sap variation and maple syrup quality |
title_full |
A systems biology approach to explore the impact of maple tree dormancy release on sap variation and maple syrup quality |
title_fullStr |
A systems biology approach to explore the impact of maple tree dormancy release on sap variation and maple syrup quality |
title_full_unstemmed |
A systems biology approach to explore the impact of maple tree dormancy release on sap variation and maple syrup quality |
title_sort |
systems biology approach to explore the impact of maple tree dormancy release on sap variation and maple syrup quality |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2018-10-01 |
description |
Abstract Maple sap is a complex nutrient matrix collected during spring to produce maple syrup. The characteristics of sap change over the production period and its composition directly impacts syrup quality. This variability could in part be attributed to changes in tree metabolism following dormancy release, but little is known about these changes in deciduous trees. Therefore, understanding the variation in sap composition associated with dormancy release could help pinpoint the causes of some defects in maple syrup. In particular, a defect known as “buddy”, is an increasing concern for the industry. This off-flavor appears around the time of bud break, hence its name. To investigate sap variation related to bud break and the buddy defect, we monitored sap variation with respect to a dormancy release index (Sbb) and syrup quality. First, we looked at variation in amino acid content during this period. We observed a shift in amino acid relative proportions associated with dormancy release and found that most of them increase rapidly near the point of bud break, correlating with changes in syrup quality. Second, we identified biological processes that respond to variation in maple sap by performing a competition assay using the barcoded Saccharomyces cerevisiae prototroph deletion collection. This untargeted approach revealed that the organic sulfur content may be responsible for the development of the buddy off-flavor, and that dormancy release is necessary for the appearance of the defect, but other factors such as microbial activity may also be contributing. |
topic |
Dormancy Release Syrup Quality Maple Trees Organic Sulfur Content Yeast Deletion Collection |
url |
https://doi.org/10.1038/s41598-018-32940-y |
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