Heterologous overexpression and mutagenesis of the human bile salt export pump (ABCB11) using DREAM (Directed REcombination-Assisted Mutagenesis).

Heterologous overexpression and mutagenesis of the human bile salt export pump (ABCB11) using DREAM (Directed REcombination-Assisted Mutagenesis).

Homologous recombination in Saccharomyces cerevisiae is a well-studied process. Here, we describe a yeast-recombination-based approach to construct and mutate plasmids containing the cDNA of the human bile salt export pump (BSEP) that has been shown to be unstable in E. coli. Using this approach, we...

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Bibliographic Details
Main Authors: Jan Stindt, Philipp Ellinger, Claudia Stross, Verena Keitel, Dieter Häussinger, Sander H J Smits, Ralf Kubitz, Lutz Schmitt
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3105083?pdf=render

Internet

http://europepmc.org/articles/PMC3105083?pdf=render

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