Variation in Mutation Spectra Among CRISPR/Cas9 Mutagenized Poplars

Variation in Mutation Spectra Among CRISPR/Cas9 Mutagenized Poplars

In an effort to produce reliably contained transgenic trees, we used the CRISPR/Cas9 system to alter three genes expected to be required for normal flowering in poplar (genus Populus). We designed synthetic guide RNAs (sgRNAs) to target the poplar homolog of the floral meristem identity gene, LEAFY...

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Bibliographic Details
Main Authors: Estefania Elorriaga, Amy L. Klocko, Cathleen Ma, Steven H. Strauss
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-05-01
Series:Frontiers in Plant Science
Subjects:
Populus
CRISPR/Cas9
site-directed-mutagenesis
LEAFY
AGAMOUS
Online Access:http://journal.frontiersin.org/article/10.3389/fpls.2018.00594/full

Internet

http://journal.frontiersin.org/article/10.3389/fpls.2018.00594/full

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