Suppressor of fused controls perinatal expansion and quiescence of future dentate adult neural stem cells
Adult hippocampal neurogenesis requires the quiescent neural stem cell (NSC) pool to persist lifelong. However, establishment and maintenance of quiescent NSC pools during development is not understood. Here, we show that Suppressor of Fused (Sufu) controls establishment of the quiescent NSC pool du...
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doaj-bdf3dfc5a4e741fb9f61ba7e48df37292021-05-05T17:32:11ZengeLife Sciences Publications LtdeLife2050-084X2019-04-01810.7554/eLife.42918Suppressor of fused controls perinatal expansion and quiescence of future dentate adult neural stem cellsHirofumi Noguchi0https://orcid.org/0000-0002-9779-4956Jesse Garcia Castillo1Kinichi Nakashima2Samuel J Pleasure3https://orcid.org/0000-0001-8599-1613Department of Neurology, University of California, San Francisco, San Francisco, United StatesDepartment of Neurology, University of California, San Francisco, San Francisco, United StatesDepartment of Stem Cell Biology and Medicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanPrograms in Neuroscience and Developmental Biology, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, San Francisco, United StatesAdult hippocampal neurogenesis requires the quiescent neural stem cell (NSC) pool to persist lifelong. However, establishment and maintenance of quiescent NSC pools during development is not understood. Here, we show that Suppressor of Fused (Sufu) controls establishment of the quiescent NSC pool during mouse dentate gyrus (DG) development by regulating Sonic Hedgehog (Shh) signaling activity. Deletion of Sufu in NSCs early in DG development decreases Shh signaling activity leading to reduced proliferation of NSCs, resulting in a small quiescent NSC pool in adult mice. We found that putative adult NSCs proliferate and increase their numbers in the first postnatal week and subsequently enter a quiescent state towards the end of the first postnatal week. In the absence of Sufu, postnatal expansion of NSCs is compromised, and NSCs prematurely become quiescent. Thus, Sufu is required for Shh signaling activity ensuring expansion and proper transition of NSC pools to quiescent states during DG development.https://elifesciences.org/articles/42918stem celldentate gyrussonic hedgehoghippocampal development |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hirofumi Noguchi Jesse Garcia Castillo Kinichi Nakashima Samuel J Pleasure |
spellingShingle |
Hirofumi Noguchi Jesse Garcia Castillo Kinichi Nakashima Samuel J Pleasure Suppressor of fused controls perinatal expansion and quiescence of future dentate adult neural stem cells eLife stem cell dentate gyrus sonic hedgehog hippocampal development |
author_facet |
Hirofumi Noguchi Jesse Garcia Castillo Kinichi Nakashima Samuel J Pleasure |
author_sort |
Hirofumi Noguchi |
title |
Suppressor of fused controls perinatal expansion and quiescence of future dentate adult neural stem cells |
title_short |
Suppressor of fused controls perinatal expansion and quiescence of future dentate adult neural stem cells |
title_full |
Suppressor of fused controls perinatal expansion and quiescence of future dentate adult neural stem cells |
title_fullStr |
Suppressor of fused controls perinatal expansion and quiescence of future dentate adult neural stem cells |
title_full_unstemmed |
Suppressor of fused controls perinatal expansion and quiescence of future dentate adult neural stem cells |
title_sort |
suppressor of fused controls perinatal expansion and quiescence of future dentate adult neural stem cells |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2019-04-01 |
description |
Adult hippocampal neurogenesis requires the quiescent neural stem cell (NSC) pool to persist lifelong. However, establishment and maintenance of quiescent NSC pools during development is not understood. Here, we show that Suppressor of Fused (Sufu) controls establishment of the quiescent NSC pool during mouse dentate gyrus (DG) development by regulating Sonic Hedgehog (Shh) signaling activity. Deletion of Sufu in NSCs early in DG development decreases Shh signaling activity leading to reduced proliferation of NSCs, resulting in a small quiescent NSC pool in adult mice. We found that putative adult NSCs proliferate and increase their numbers in the first postnatal week and subsequently enter a quiescent state towards the end of the first postnatal week. In the absence of Sufu, postnatal expansion of NSCs is compromised, and NSCs prematurely become quiescent. Thus, Sufu is required for Shh signaling activity ensuring expansion and proper transition of NSC pools to quiescent states during DG development. |
topic |
stem cell dentate gyrus sonic hedgehog hippocampal development |
url |
https://elifesciences.org/articles/42918 |
work_keys_str_mv |
AT hirofuminoguchi suppressoroffusedcontrolsperinatalexpansionandquiescenceoffuturedentateadultneuralstemcells AT jessegarciacastillo suppressoroffusedcontrolsperinatalexpansionandquiescenceoffuturedentateadultneuralstemcells AT kinichinakashima suppressoroffusedcontrolsperinatalexpansionandquiescenceoffuturedentateadultneuralstemcells AT samueljpleasure suppressoroffusedcontrolsperinatalexpansionandquiescenceoffuturedentateadultneuralstemcells |
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1721459241484550144 |