A time-dependent, two-step binding mode of the nitro dye flavianic acid to trypsin in acid media

Synthetic dyes bind to proteins causing selective coprecipitation of the complexes in acid aqueous solution by a process of reversible denaturation that can be used as an alternative method for protein fractionation. The events that occur before precipitation were investigated by equilibrium dialysi...

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Bibliographic Details
Main Authors: J.M. Schneedorf, M.M. Santoro, R.E. Lovrien, M. Mares-Guia
Format: Article
Language:English
Published: Associação Brasileira de Divulgação Científica 2001-08-01
Series:Brazilian Journal of Medical and Biological Research
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800012