Reliability of quantitative real-time PCR for bacterial detection in cystic fibrosis airway specimens.
The cystic fibrosis (CF) airway microbiome is complex; polymicrobial infections are common, and the presence of fastidious bacteria including anaerobes make culture-based diagnosis challenging. Quantitative real-time PCR (qPCR) offers a culture-independent method for bacterial quantification that ma...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2010-11-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC2994853?pdf=render |