Solid Lipid Nanoparticles Loaded with Glucocorticoids Protect Auditory Cells from Cisplatin-Induced Ototoxicity

Cisplatin is a chemotherapeutic agent that causes the irreversible death of auditory sensory cells, leading to hearing loss. Local administration of cytoprotective drugs is a potentially better option co-therapy for cisplatin, but there are strong limitations due to the difficulty of accessing the i...

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Main Authors: Blanca Cervantes, Lide Arana, Silvia Murillo-Cuesta, Marina Bruno, Itziar Alkorta, Isabel Varela-Nieto
Format: Article
Language:English
Published: MDPI AG 2019-09-01
Series:Journal of Clinical Medicine
Subjects:
Online Access:https://www.mdpi.com/2077-0383/8/9/1464
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spelling doaj-bec1a73db08141a1b10c20d0ae49cb792020-11-25T02:03:25ZengMDPI AGJournal of Clinical Medicine2077-03832019-09-0189146410.3390/jcm8091464jcm8091464Solid Lipid Nanoparticles Loaded with Glucocorticoids Protect Auditory Cells from Cisplatin-Induced OtotoxicityBlanca Cervantes0Lide Arana1Silvia Murillo-Cuesta2Marina Bruno3Itziar Alkorta4Isabel Varela-Nieto5Institute for Biomedical Research “Alberto Sols” (IIBM), Spanish National Research Council-Autonomous University of Madrid (CSIC-UAM), 28029 Madrid, SpainDepartment of Biochemistry and Molecular Biology, University of the Basque Country (UPV/EHU), Barrio Sarriena S/N, 48080 Leioa, SpainInstitute for Biomedical Research “Alberto Sols” (IIBM), Spanish National Research Council-Autonomous University of Madrid (CSIC-UAM), 28029 Madrid, SpainInstitute for Biomedical Research “Alberto Sols” (IIBM), Spanish National Research Council-Autonomous University of Madrid (CSIC-UAM), 28029 Madrid, SpainDepartment of Biochemistry and Molecular Biology, University of the Basque Country (UPV/EHU), Barrio Sarriena S/N, 48080 Leioa, SpainInstitute for Biomedical Research “Alberto Sols” (IIBM), Spanish National Research Council-Autonomous University of Madrid (CSIC-UAM), 28029 Madrid, SpainCisplatin is a chemotherapeutic agent that causes the irreversible death of auditory sensory cells, leading to hearing loss. Local administration of cytoprotective drugs is a potentially better option co-therapy for cisplatin, but there are strong limitations due to the difficulty of accessing the inner ear. The use of nanocarriers for the efficient delivery of drugs to auditory cells is a novel approach for this problem. Solid lipid nanoparticles (SLNs) are biodegradable and biocompatible nanocarriers with low solubility in aqueous media. We show here that stearic acid-based SLNs have the adequate particle size, polydispersity index and ζ-potential, to be considered optimal nanocarriers for drug delivery. Stearic acid-based SLNs were loaded with the fluorescent probe rhodamine to show that they are efficiently incorporated by auditory HEI-OC1 (House Ear Institute-Organ of Corti 1) cells. SLNs were not ototoxic over a wide dose range. Glucocorticoids are used to decrease cisplatin-induced ototoxicity. Therefore, to test SLNs’ drug delivery efficiency, dexamethasone and hydrocortisone were tested either alone or loaded into SLNs and tested in a cisplatin-induced ototoxicity in vitro assay. Our results indicate that the encapsulation in SLNs increases the protective effect of low doses of hydrocortisone and lengthens the survival of HEI-OC1 cells treated with cisplatin.https://www.mdpi.com/2077-0383/8/9/1464nanocarriersdexamethasonehydrocortisoneotic protectionstearic acid-based solid lipid nanoparticle (SLN)
collection DOAJ
language English
format Article
sources DOAJ
author Blanca Cervantes
Lide Arana
Silvia Murillo-Cuesta
Marina Bruno
Itziar Alkorta
Isabel Varela-Nieto
spellingShingle Blanca Cervantes
Lide Arana
Silvia Murillo-Cuesta
Marina Bruno
Itziar Alkorta
Isabel Varela-Nieto
Solid Lipid Nanoparticles Loaded with Glucocorticoids Protect Auditory Cells from Cisplatin-Induced Ototoxicity
Journal of Clinical Medicine
nanocarriers
dexamethasone
hydrocortisone
otic protection
stearic acid-based solid lipid nanoparticle (SLN)
author_facet Blanca Cervantes
Lide Arana
Silvia Murillo-Cuesta
Marina Bruno
Itziar Alkorta
Isabel Varela-Nieto
author_sort Blanca Cervantes
title Solid Lipid Nanoparticles Loaded with Glucocorticoids Protect Auditory Cells from Cisplatin-Induced Ototoxicity
title_short Solid Lipid Nanoparticles Loaded with Glucocorticoids Protect Auditory Cells from Cisplatin-Induced Ototoxicity
title_full Solid Lipid Nanoparticles Loaded with Glucocorticoids Protect Auditory Cells from Cisplatin-Induced Ototoxicity
title_fullStr Solid Lipid Nanoparticles Loaded with Glucocorticoids Protect Auditory Cells from Cisplatin-Induced Ototoxicity
title_full_unstemmed Solid Lipid Nanoparticles Loaded with Glucocorticoids Protect Auditory Cells from Cisplatin-Induced Ototoxicity
title_sort solid lipid nanoparticles loaded with glucocorticoids protect auditory cells from cisplatin-induced ototoxicity
publisher MDPI AG
series Journal of Clinical Medicine
issn 2077-0383
publishDate 2019-09-01
description Cisplatin is a chemotherapeutic agent that causes the irreversible death of auditory sensory cells, leading to hearing loss. Local administration of cytoprotective drugs is a potentially better option co-therapy for cisplatin, but there are strong limitations due to the difficulty of accessing the inner ear. The use of nanocarriers for the efficient delivery of drugs to auditory cells is a novel approach for this problem. Solid lipid nanoparticles (SLNs) are biodegradable and biocompatible nanocarriers with low solubility in aqueous media. We show here that stearic acid-based SLNs have the adequate particle size, polydispersity index and ζ-potential, to be considered optimal nanocarriers for drug delivery. Stearic acid-based SLNs were loaded with the fluorescent probe rhodamine to show that they are efficiently incorporated by auditory HEI-OC1 (House Ear Institute-Organ of Corti 1) cells. SLNs were not ototoxic over a wide dose range. Glucocorticoids are used to decrease cisplatin-induced ototoxicity. Therefore, to test SLNs’ drug delivery efficiency, dexamethasone and hydrocortisone were tested either alone or loaded into SLNs and tested in a cisplatin-induced ototoxicity in vitro assay. Our results indicate that the encapsulation in SLNs increases the protective effect of low doses of hydrocortisone and lengthens the survival of HEI-OC1 cells treated with cisplatin.
topic nanocarriers
dexamethasone
hydrocortisone
otic protection
stearic acid-based solid lipid nanoparticle (SLN)
url https://www.mdpi.com/2077-0383/8/9/1464
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AT silviamurillocuesta solidlipidnanoparticlesloadedwithglucocorticoidsprotectauditorycellsfromcisplatininducedototoxicity
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