An optimized genome-wide, virus-free CRISPR screen for mammalian cells

An optimized genome-wide, virus-free CRISPR screen for mammalian cells

Summary: Pooled CRISPR screens have been widely applied to mammalian and other organisms to elucidate the interplay between genes and phenotypes of interest. The most popular method for delivering the CRISPR components into mammalian cells is lentivirus based. However, because lentivirus is not alwa...

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Bibliographic Details
Main Authors: Kai Xiong, Karen Julie la Cour Karottki, Hooman Hefzi, Songyuan Li, Lise Marie Grav, Shangzhong Li, Philipp Spahn, Jae Seong Lee, Ildze Ventina, Gyun Min Lee, Nathan E. Lewis, Lasse Ebdrup Pedersen
Format: Article
Language:English
Published: Elsevier 2021-08-01
Series:Cell Reports: Methods
Subjects:
CRISPR
Cas9
pooled screen
virus free
genome wide
CHO
Online Access:http://www.sciencedirect.com/science/article/pii/S2667237521001107

Internet

http://www.sciencedirect.com/science/article/pii/S2667237521001107

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