Decreased dicer expression enhances SRP-mediated protein targeting.
We have shown that Dicer processes 7SL RNA into different fragments ranging from ∼20 to more than 200 nucleotides. Here we addressed the molecular functions of these 7SL RNA fragments and found that some of them functioned as dominant-negative regulators of the full-length 7SL RNA, interfering with...
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doaj-bf22ac33ced44a40941db72c1f5a15642020-11-25T02:42:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0182e5695010.1371/journal.pone.0056950Decreased dicer expression enhances SRP-mediated protein targeting.Yong-Feng RenGuiling LiYong-Feng XueXue-Jiao ZhangYi-Jiang SongLu LvJianmin WuYu-Xiao FangYu-Qun WangKe-Qing ShiYong-Ping ChenKai-Fu TangWe have shown that Dicer processes 7SL RNA into different fragments ranging from ∼20 to more than 200 nucleotides. Here we addressed the molecular functions of these 7SL RNA fragments and found that some of them functioned as dominant-negative regulators of the full-length 7SL RNA, interfering with signal recognition particle (SRP) complex formation. Transfection of these 7SL RNA fragments inhibited the expression of cell surface glycoproteins, the targeting of a reporter protein to the endoplasmic reticulum, and the secretion of secreted alkaline phosphatase. These results suggest that some Dicer-processed 7SL RNA fragments interfered with SRP-mediated protein targeting. Moreover, we showed that Dicer knockdown enhanced SRP-mediated protein targeting and that transfection of a mixture of the 7SL RNA fragments partially restored this effect. Our data indicate that Dicer can fine-tune the efficiency of SRP-mediated protein targeting via processing a proportion of 7SL RNA into fragments of different lengths.http://europepmc.org/articles/PMC3585229?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yong-Feng Ren Guiling Li Yong-Feng Xue Xue-Jiao Zhang Yi-Jiang Song Lu Lv Jianmin Wu Yu-Xiao Fang Yu-Qun Wang Ke-Qing Shi Yong-Ping Chen Kai-Fu Tang |
spellingShingle |
Yong-Feng Ren Guiling Li Yong-Feng Xue Xue-Jiao Zhang Yi-Jiang Song Lu Lv Jianmin Wu Yu-Xiao Fang Yu-Qun Wang Ke-Qing Shi Yong-Ping Chen Kai-Fu Tang Decreased dicer expression enhances SRP-mediated protein targeting. PLoS ONE |
author_facet |
Yong-Feng Ren Guiling Li Yong-Feng Xue Xue-Jiao Zhang Yi-Jiang Song Lu Lv Jianmin Wu Yu-Xiao Fang Yu-Qun Wang Ke-Qing Shi Yong-Ping Chen Kai-Fu Tang |
author_sort |
Yong-Feng Ren |
title |
Decreased dicer expression enhances SRP-mediated protein targeting. |
title_short |
Decreased dicer expression enhances SRP-mediated protein targeting. |
title_full |
Decreased dicer expression enhances SRP-mediated protein targeting. |
title_fullStr |
Decreased dicer expression enhances SRP-mediated protein targeting. |
title_full_unstemmed |
Decreased dicer expression enhances SRP-mediated protein targeting. |
title_sort |
decreased dicer expression enhances srp-mediated protein targeting. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
We have shown that Dicer processes 7SL RNA into different fragments ranging from ∼20 to more than 200 nucleotides. Here we addressed the molecular functions of these 7SL RNA fragments and found that some of them functioned as dominant-negative regulators of the full-length 7SL RNA, interfering with signal recognition particle (SRP) complex formation. Transfection of these 7SL RNA fragments inhibited the expression of cell surface glycoproteins, the targeting of a reporter protein to the endoplasmic reticulum, and the secretion of secreted alkaline phosphatase. These results suggest that some Dicer-processed 7SL RNA fragments interfered with SRP-mediated protein targeting. Moreover, we showed that Dicer knockdown enhanced SRP-mediated protein targeting and that transfection of a mixture of the 7SL RNA fragments partially restored this effect. Our data indicate that Dicer can fine-tune the efficiency of SRP-mediated protein targeting via processing a proportion of 7SL RNA into fragments of different lengths. |
url |
http://europepmc.org/articles/PMC3585229?pdf=render |
work_keys_str_mv |
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