Antioxidative Activity and Inhibition of Key Enzymes Linked to Type-2 Diabetes (α-Glucosidase and α-Amylase) by Khaya Senegalensis

This study evaluated the in vitro antioxidative activity of Khaya senegalensis extracts and inhibitory effects of some solvent fractions on α-glucosidase and α-amylase activities. The stem bark, root and leaf samples of the plant were sequentially extracted with ethyl acetate, ethanol and water and...

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Main Authors: Ibrahim Mohammed Auwal, Koorbanally Neil Anthony, Islam Md. Shahidul
Format: Article
Language:English
Published: Sciendo 2014-09-01
Series:Acta Pharmaceutica
Subjects:
Online Access:https://doi.org/10.2478/acph-2014-0025
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spelling doaj-bf38f46a397f48c790dcd074be7c56cc2021-09-06T19:40:58ZengSciendoActa Pharmaceutica1846-95582014-09-0164331132410.2478/acph-2014-0025acph-2014-0025Antioxidative Activity and Inhibition of Key Enzymes Linked to Type-2 Diabetes (α-Glucosidase and α-Amylase) by Khaya SenegalensisIbrahim Mohammed Auwal0Koorbanally Neil Anthony1Islam Md. Shahidul2School of Life Sciences and Department of Biochemistry Ahmadu Bello University, Zaria NigeriaSchool of Chemistry and Physics University of KwaZulu-Natal (Westville Campus) Durban, 4000, South AfricaSchool of Life SciencesThis study evaluated the in vitro antioxidative activity of Khaya senegalensis extracts and inhibitory effects of some solvent fractions on α-glucosidase and α-amylase activities. The stem bark, root and leaf samples of the plant were sequentially extracted with ethyl acetate, ethanol and water and then tested for antioxidative activity. Our findings revealed that the ethanolic extract of the root had the highest antioxidative activity. Solvent-solvent fractionation of the root ethanolic extract yielded a butanol fraction that showed higher antioxidative activity than other fractions. Furthermore, the butanol fraction had significantly higher (p < 0.05) α-glucosidase and α-amylase inhibitory activities with IC50 values of 2.89 ± 0.46 and 97.51 ± 5.72 μg mL-1, respectively. Enzyme kinetic studies indicated that the butanol fraction is a non-competitive inhibitor for α-glucosidase with an inhibition binding constant Ki of 1.30 μg mL-1 and a competitive inhibitor of α-amylase with a Ki of 7.50 μg mL-1. GC-MS analysis revealed that the butanol fraction contained two chromones, p-anilinophenol and 3-ethyl-5-(3- ethyl-(3H)-benzothiazol-2-ylidene)-2-(p-tolylvinylamino)- 4-thiazolidinone. Data obtained in the study suggest that the butanol fraction derived from the ethanolic extract of K. senegalensis root possessed excellent antioxidative as well as α-glucosidase and a-amylase inhibitory activities while chromones and/or p-anilinophenol could be the main bioactive compounds responsible for the observed activities.https://doi.org/10.2478/acph-2014-0025khaya senegalensis (meliaceae)type-2 diabetesα-glucosidaseα-amylaseoxidative stress
collection DOAJ
language English
format Article
sources DOAJ
author Ibrahim Mohammed Auwal
Koorbanally Neil Anthony
Islam Md. Shahidul
spellingShingle Ibrahim Mohammed Auwal
Koorbanally Neil Anthony
Islam Md. Shahidul
Antioxidative Activity and Inhibition of Key Enzymes Linked to Type-2 Diabetes (α-Glucosidase and α-Amylase) by Khaya Senegalensis
Acta Pharmaceutica
khaya senegalensis (meliaceae)
type-2 diabetes
α-glucosidase
α-amylase
oxidative stress
author_facet Ibrahim Mohammed Auwal
Koorbanally Neil Anthony
Islam Md. Shahidul
author_sort Ibrahim Mohammed Auwal
title Antioxidative Activity and Inhibition of Key Enzymes Linked to Type-2 Diabetes (α-Glucosidase and α-Amylase) by Khaya Senegalensis
title_short Antioxidative Activity and Inhibition of Key Enzymes Linked to Type-2 Diabetes (α-Glucosidase and α-Amylase) by Khaya Senegalensis
title_full Antioxidative Activity and Inhibition of Key Enzymes Linked to Type-2 Diabetes (α-Glucosidase and α-Amylase) by Khaya Senegalensis
title_fullStr Antioxidative Activity and Inhibition of Key Enzymes Linked to Type-2 Diabetes (α-Glucosidase and α-Amylase) by Khaya Senegalensis
title_full_unstemmed Antioxidative Activity and Inhibition of Key Enzymes Linked to Type-2 Diabetes (α-Glucosidase and α-Amylase) by Khaya Senegalensis
title_sort antioxidative activity and inhibition of key enzymes linked to type-2 diabetes (α-glucosidase and α-amylase) by khaya senegalensis
publisher Sciendo
series Acta Pharmaceutica
issn 1846-9558
publishDate 2014-09-01
description This study evaluated the in vitro antioxidative activity of Khaya senegalensis extracts and inhibitory effects of some solvent fractions on α-glucosidase and α-amylase activities. The stem bark, root and leaf samples of the plant were sequentially extracted with ethyl acetate, ethanol and water and then tested for antioxidative activity. Our findings revealed that the ethanolic extract of the root had the highest antioxidative activity. Solvent-solvent fractionation of the root ethanolic extract yielded a butanol fraction that showed higher antioxidative activity than other fractions. Furthermore, the butanol fraction had significantly higher (p < 0.05) α-glucosidase and α-amylase inhibitory activities with IC50 values of 2.89 ± 0.46 and 97.51 ± 5.72 μg mL-1, respectively. Enzyme kinetic studies indicated that the butanol fraction is a non-competitive inhibitor for α-glucosidase with an inhibition binding constant Ki of 1.30 μg mL-1 and a competitive inhibitor of α-amylase with a Ki of 7.50 μg mL-1. GC-MS analysis revealed that the butanol fraction contained two chromones, p-anilinophenol and 3-ethyl-5-(3- ethyl-(3H)-benzothiazol-2-ylidene)-2-(p-tolylvinylamino)- 4-thiazolidinone. Data obtained in the study suggest that the butanol fraction derived from the ethanolic extract of K. senegalensis root possessed excellent antioxidative as well as α-glucosidase and a-amylase inhibitory activities while chromones and/or p-anilinophenol could be the main bioactive compounds responsible for the observed activities.
topic khaya senegalensis (meliaceae)
type-2 diabetes
α-glucosidase
α-amylase
oxidative stress
url https://doi.org/10.2478/acph-2014-0025
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