Poultry and Wild Birds as a Reservoir of CMY-2 Producing <i>Escherichia coli</i>: The First Large-Scale Study in Greece
Resistance mediated by β-lactamases is a globally spread menace. The aim of the present study was to determine the occurrence of <i>Escherichia coli</i> producing plasmid-encoded AmpC β-lactamases (pAmpC) in animals. Fecal samples from chickens (n = 159), cattle (n = 104), pigs (n = 214)...
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doaj-bf3f60682e544839800160d4653e3f982021-02-27T00:01:14ZengMDPI AGAntibiotics2079-63822021-02-011023523510.3390/antibiotics10030235Poultry and Wild Birds as a Reservoir of CMY-2 Producing <i>Escherichia coli</i>: The First Large-Scale Study in GreeceZoi Athanasakopoulou0Katerina Tsilipounidaki1Marina Sofia2Dimitris C. Chatzopoulos3Alexios Giannakopoulos4Ioannis Karakousis5Vassilios Giannakis6Vassiliki Spyrou7Antonia Touloudi8Maria Satra9Dimitrios Galamatis10Vassilis Diamantopoulos11Spyridoula Mpellou12Efthymia Petinaki13Charalambos Billinis14Faculty of Veterinary Science, University of Thessaly, 43100 Karditsa, GreeceFaculty of Medicine, University of Thessaly, 41500 Larissa, GreeceFaculty of Veterinary Science, University of Thessaly, 43100 Karditsa, GreeceFaculty of Veterinary Science, University of Thessaly, 43100 Karditsa, GreeceFaculty of Veterinary Science, University of Thessaly, 43100 Karditsa, GreeceElanco Hellas S.A.C.I., 15231 Athens, GreeceElanco Hellas S.A.C.I., 15231 Athens, GreeceFaculty of Animal Science, University of Thessaly, 41110 Larissa, GreeceFaculty of Veterinary Science, University of Thessaly, 43100 Karditsa, GreeceFaculty of Public and Integrated Health, University of Thessaly, 43100 Karditsa, GreeceHellenic Agricultural Organization DIMITRA (ELGO DIMITRA), 57001 Thessaloniki, GreeceDirectorate of Public Health, Prefecture of Peloponnese, 22132 Tripoli, GreeceBioefarmoges Eleftheriou LP-Integrated Mosquito Control, 19007 Marathon, GreeceFaculty of Medicine, University of Thessaly, 41500 Larissa, GreeceFaculty of Veterinary Science, University of Thessaly, 43100 Karditsa, GreeceResistance mediated by β-lactamases is a globally spread menace. The aim of the present study was to determine the occurrence of <i>Escherichia coli</i> producing plasmid-encoded AmpC β-lactamases (pAmpC) in animals. Fecal samples from chickens (n = 159), cattle (n = 104), pigs (n = 214), and various wild bird species (n = 168), collected from different Greek regions during 2018–2020, were screened for the presence of pAmpC-encoding genes. Thirteen <i>E. coli</i> displaying resistance to third-generation cephalosporins and a positive AmpC confirmation test were detected. <i>bla</i><sub>CMY-2</sub> was the sole pAmpC gene identified in 12 chickens’ and 1 wild bird (Eurasian magpie) isolates and was in all cases linked to an upstream ISE<i>cp1</i>-like element. The isolates were classified into five different sequence types: ST131, ST117, ST155, ST429, and ST1415. Four chickens’ stains were assigned to ST131, while five chickens’ strains and the one from the Eurasian magpie belonged to ST117. Seven pAmpC isolates co-harbored genes conferring resistance to tetracyclines (<i>tetM, tetB, tetC, tetD</i>), 3 carried sulfonamide resistance genes (<i>sul</i>I and <i>sul</i>II), and 10 displayed mutations in the quinolone resistance-determining regions of <i>gyrA</i> (S83L+D87N) and <i>parC</i> (S80I+E84V). This report provides evidence of pAmpC dissemination, describing for the first time the presence of CMY-2 in chickens and wild birds from Greece.https://www.mdpi.com/2079-6382/10/3/235<i>Escherichia coli</i>AmpC β-lactamasesantimicrobial resistanceCMY-2 typeISE<i>cp1</i>chickens |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zoi Athanasakopoulou Katerina Tsilipounidaki Marina Sofia Dimitris C. Chatzopoulos Alexios Giannakopoulos Ioannis Karakousis Vassilios Giannakis Vassiliki Spyrou Antonia Touloudi Maria Satra Dimitrios Galamatis Vassilis Diamantopoulos Spyridoula Mpellou Efthymia Petinaki Charalambos Billinis |
spellingShingle |
Zoi Athanasakopoulou Katerina Tsilipounidaki Marina Sofia Dimitris C. Chatzopoulos Alexios Giannakopoulos Ioannis Karakousis Vassilios Giannakis Vassiliki Spyrou Antonia Touloudi Maria Satra Dimitrios Galamatis Vassilis Diamantopoulos Spyridoula Mpellou Efthymia Petinaki Charalambos Billinis Poultry and Wild Birds as a Reservoir of CMY-2 Producing <i>Escherichia coli</i>: The First Large-Scale Study in Greece Antibiotics <i>Escherichia coli</i> AmpC β-lactamases antimicrobial resistance CMY-2 type ISE<i>cp1</i> chickens |
author_facet |
Zoi Athanasakopoulou Katerina Tsilipounidaki Marina Sofia Dimitris C. Chatzopoulos Alexios Giannakopoulos Ioannis Karakousis Vassilios Giannakis Vassiliki Spyrou Antonia Touloudi Maria Satra Dimitrios Galamatis Vassilis Diamantopoulos Spyridoula Mpellou Efthymia Petinaki Charalambos Billinis |
author_sort |
Zoi Athanasakopoulou |
title |
Poultry and Wild Birds as a Reservoir of CMY-2 Producing <i>Escherichia coli</i>: The First Large-Scale Study in Greece |
title_short |
Poultry and Wild Birds as a Reservoir of CMY-2 Producing <i>Escherichia coli</i>: The First Large-Scale Study in Greece |
title_full |
Poultry and Wild Birds as a Reservoir of CMY-2 Producing <i>Escherichia coli</i>: The First Large-Scale Study in Greece |
title_fullStr |
Poultry and Wild Birds as a Reservoir of CMY-2 Producing <i>Escherichia coli</i>: The First Large-Scale Study in Greece |
title_full_unstemmed |
Poultry and Wild Birds as a Reservoir of CMY-2 Producing <i>Escherichia coli</i>: The First Large-Scale Study in Greece |
title_sort |
poultry and wild birds as a reservoir of cmy-2 producing <i>escherichia coli</i>: the first large-scale study in greece |
publisher |
MDPI AG |
series |
Antibiotics |
issn |
2079-6382 |
publishDate |
2021-02-01 |
description |
Resistance mediated by β-lactamases is a globally spread menace. The aim of the present study was to determine the occurrence of <i>Escherichia coli</i> producing plasmid-encoded AmpC β-lactamases (pAmpC) in animals. Fecal samples from chickens (n = 159), cattle (n = 104), pigs (n = 214), and various wild bird species (n = 168), collected from different Greek regions during 2018–2020, were screened for the presence of pAmpC-encoding genes. Thirteen <i>E. coli</i> displaying resistance to third-generation cephalosporins and a positive AmpC confirmation test were detected. <i>bla</i><sub>CMY-2</sub> was the sole pAmpC gene identified in 12 chickens’ and 1 wild bird (Eurasian magpie) isolates and was in all cases linked to an upstream ISE<i>cp1</i>-like element. The isolates were classified into five different sequence types: ST131, ST117, ST155, ST429, and ST1415. Four chickens’ stains were assigned to ST131, while five chickens’ strains and the one from the Eurasian magpie belonged to ST117. Seven pAmpC isolates co-harbored genes conferring resistance to tetracyclines (<i>tetM, tetB, tetC, tetD</i>), 3 carried sulfonamide resistance genes (<i>sul</i>I and <i>sul</i>II), and 10 displayed mutations in the quinolone resistance-determining regions of <i>gyrA</i> (S83L+D87N) and <i>parC</i> (S80I+E84V). This report provides evidence of pAmpC dissemination, describing for the first time the presence of CMY-2 in chickens and wild birds from Greece. |
topic |
<i>Escherichia coli</i> AmpC β-lactamases antimicrobial resistance CMY-2 type ISE<i>cp1</i> chickens |
url |
https://www.mdpi.com/2079-6382/10/3/235 |
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