A protocol for characterization of extremely preterm infant gut microbiota in double-blind clinical trials

A protocol for characterization of extremely preterm infant gut microbiota in double-blind clinical trials

Summary: 16S rRNA gene sequencing enables microbial community profiling, but recovering fecal DNA from extremely premature infants is challenging. Here, we describe an optimized protocol for fecal DNA isolation, library preparation for 16S rRNA gene sequencing, taxonomy assignation, and statistical...

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Bibliographic Details
Main Authors: Magalí Martí, Johanne E. Spreckels, Maria C. Jenmalm, Thomas Abrahamsson
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Health Sciences
Clinical Protocol
Sequencing
Microbiology
Molecular Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721003592

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http://www.sciencedirect.com/science/article/pii/S2666166721003592

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