Droplet Microfluidics Approach for Single-DNA Molecule Amplification and Condensation into DNA-Magnesium-Pyrophosphate Particles

Droplet Microfluidics Approach for Single-DNA Molecule Amplification and Condensation into DNA-Magnesium-Pyrophosphate Particles

Protein expression in vitro has broad applications in directed evolution, synthetic biology, proteomics and drug screening. However, most of the in vitro expression systems rely on relatively high DNA template concentrations to obtain sufficient amounts of proteins, making it harder to perform in vi...

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Bibliographic Details
Main Authors: Greta Zubaite, Karolis Simutis, Robertas Galinis, Valdemaras Milkus, Vaidotas Kiseliovas, Linas Mazutis
Format: Article
Language:English
Published: MDPI AG 2017-02-01
Series:Micromachines
Subjects:
in vitro expression
DNA condensation
droplet microfluidics
phi29 DNA polymerase
Online Access:http://www.mdpi.com/2072-666X/8/2/62

Internet

http://www.mdpi.com/2072-666X/8/2/62

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