Cell proliferation and interferon-gamma response to recombinant MBP-3, NarL, MT-10.3, and 16kDa Mycobacterium tuberculosis antigens in Brazilian tuberculosis patients
Human pulmonary tuberculosis (TB) is a worldwide public health problem. In resistant individuals, control of the infection mainly requires development of a Th1 cell immune response with production of cytokines, of which interferon-gamma (IFN-gamma)plays an important role. Several antigens from Mycob...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Instituto Oswaldo Cruz, Ministério da Saúde
2006-12-01
|
Series: | Memórias do Instituto Oswaldo Cruz. |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000800006 |
id |
doaj-bfa224c810dd468e969e09b866622fdd |
---|---|
record_format |
Article |
spelling |
doaj-bfa224c810dd468e969e09b866622fdd2020-11-24T21:27:40ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.0074-02761678-80602006-12-01101885786110.1590/S0074-02762006000800006Cell proliferation and interferon-gamma response to recombinant MBP-3, NarL, MT-10.3, and 16kDa Mycobacterium tuberculosis antigens in Brazilian tuberculosis patientsRicardo Candido Oliveira TavaresJorge SalgadoValéria Barbosa MoreiraMônica Antonia S FerreiraFernanda Carvalho Queoz MelloJanaína AW LeungMahavir SinghLeila de Souza FonsecaMaria Helena Feres SaadHuman pulmonary tuberculosis (TB) is a worldwide public health problem. In resistant individuals, control of the infection mainly requires development of a Th1 cell immune response with production of cytokines, of which interferon-gamma (IFN-gamma)plays an important role. Several antigens from Mycobacterium tuberculosis complex has been described for use in vaccine development or for diagnostic purposes, however little evaluation has been done in endemic area for TB. The proliferative and IFN-gamma human T cell immune responses, to four recombinant proteins (MBP-3, NarL, MT-10.3, 16 kDa) and PPD, of 38 Brazilian TB patients (6 untreated and 32 treated) and 67 controls (38 positive and 29 negative tuberculin skin test - TST) were compared. The highest reactivity mean rate was obtained with PPD followed by 16 kDa in TB patients. While most of the patients (87%) and controls (> 64%) respond to the PPD, 16kDa was more specifically recognized (> 21%) although less sensitive (54%). When TB patients were divided according to treatment status, opposite to PPD, higher average level of IFN-gamma was induced by 16kDa in untreated (505 pg/ml) compared to treated TB patients and TST+ (269.8 pg/ml x 221.6pg/ml, respectively), although the difference was not significant. These data show that in contrast with the other recombinant proteins, the stimulatory potency of 16kDa to induce proliferative and INF-gamma response was more effective and is more recognized by active TB untreated patients, eliciting in control individuals a more selective immune response than PPD.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000800006tuberculosis16 kDaMT10.3interferon-gammaimmune response |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ricardo Candido Oliveira Tavares Jorge Salgado Valéria Barbosa Moreira Mônica Antonia S Ferreira Fernanda Carvalho Queoz Mello Janaína AW Leung Mahavir Singh Leila de Souza Fonseca Maria Helena Feres Saad |
spellingShingle |
Ricardo Candido Oliveira Tavares Jorge Salgado Valéria Barbosa Moreira Mônica Antonia S Ferreira Fernanda Carvalho Queoz Mello Janaína AW Leung Mahavir Singh Leila de Souza Fonseca Maria Helena Feres Saad Cell proliferation and interferon-gamma response to recombinant MBP-3, NarL, MT-10.3, and 16kDa Mycobacterium tuberculosis antigens in Brazilian tuberculosis patients Memórias do Instituto Oswaldo Cruz. tuberculosis 16 kDa MT10.3 interferon-gamma immune response |
author_facet |
Ricardo Candido Oliveira Tavares Jorge Salgado Valéria Barbosa Moreira Mônica Antonia S Ferreira Fernanda Carvalho Queoz Mello Janaína AW Leung Mahavir Singh Leila de Souza Fonseca Maria Helena Feres Saad |
author_sort |
Ricardo Candido Oliveira Tavares |
title |
Cell proliferation and interferon-gamma response to recombinant MBP-3, NarL, MT-10.3, and 16kDa Mycobacterium tuberculosis antigens in Brazilian tuberculosis patients |
title_short |
Cell proliferation and interferon-gamma response to recombinant MBP-3, NarL, MT-10.3, and 16kDa Mycobacterium tuberculosis antigens in Brazilian tuberculosis patients |
title_full |
Cell proliferation and interferon-gamma response to recombinant MBP-3, NarL, MT-10.3, and 16kDa Mycobacterium tuberculosis antigens in Brazilian tuberculosis patients |
title_fullStr |
Cell proliferation and interferon-gamma response to recombinant MBP-3, NarL, MT-10.3, and 16kDa Mycobacterium tuberculosis antigens in Brazilian tuberculosis patients |
title_full_unstemmed |
Cell proliferation and interferon-gamma response to recombinant MBP-3, NarL, MT-10.3, and 16kDa Mycobacterium tuberculosis antigens in Brazilian tuberculosis patients |
title_sort |
cell proliferation and interferon-gamma response to recombinant mbp-3, narl, mt-10.3, and 16kda mycobacterium tuberculosis antigens in brazilian tuberculosis patients |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
series |
Memórias do Instituto Oswaldo Cruz. |
issn |
0074-0276 1678-8060 |
publishDate |
2006-12-01 |
description |
Human pulmonary tuberculosis (TB) is a worldwide public health problem. In resistant individuals, control of the infection mainly requires development of a Th1 cell immune response with production of cytokines, of which interferon-gamma (IFN-gamma)plays an important role. Several antigens from Mycobacterium tuberculosis complex has been described for use in vaccine development or for diagnostic purposes, however little evaluation has been done in endemic area for TB. The proliferative and IFN-gamma human T cell immune responses, to four recombinant proteins (MBP-3, NarL, MT-10.3, 16 kDa) and PPD, of 38 Brazilian TB patients (6 untreated and 32 treated) and 67 controls (38 positive and 29 negative tuberculin skin test - TST) were compared. The highest reactivity mean rate was obtained with PPD followed by 16 kDa in TB patients. While most of the patients (87%) and controls (> 64%) respond to the PPD, 16kDa was more specifically recognized (> 21%) although less sensitive (54%). When TB patients were divided according to treatment status, opposite to PPD, higher average level of IFN-gamma was induced by 16kDa in untreated (505 pg/ml) compared to treated TB patients and TST+ (269.8 pg/ml x 221.6pg/ml, respectively), although the difference was not significant. These data show that in contrast with the other recombinant proteins, the stimulatory potency of 16kDa to induce proliferative and INF-gamma response was more effective and is more recognized by active TB untreated patients, eliciting in control individuals a more selective immune response than PPD. |
topic |
tuberculosis 16 kDa MT10.3 interferon-gamma immune response |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000800006 |
work_keys_str_mv |
AT ricardocandidooliveiratavares cellproliferationandinterferongammaresponsetorecombinantmbp3narlmt103and16kdamycobacteriumtuberculosisantigensinbraziliantuberculosispatients AT jorgesalgado cellproliferationandinterferongammaresponsetorecombinantmbp3narlmt103and16kdamycobacteriumtuberculosisantigensinbraziliantuberculosispatients AT valeriabarbosamoreira cellproliferationandinterferongammaresponsetorecombinantmbp3narlmt103and16kdamycobacteriumtuberculosisantigensinbraziliantuberculosispatients AT monicaantoniasferreira cellproliferationandinterferongammaresponsetorecombinantmbp3narlmt103and16kdamycobacteriumtuberculosisantigensinbraziliantuberculosispatients AT fernandacarvalhoqueozmello cellproliferationandinterferongammaresponsetorecombinantmbp3narlmt103and16kdamycobacteriumtuberculosisantigensinbraziliantuberculosispatients AT janainaawleung cellproliferationandinterferongammaresponsetorecombinantmbp3narlmt103and16kdamycobacteriumtuberculosisantigensinbraziliantuberculosispatients AT mahavirsingh cellproliferationandinterferongammaresponsetorecombinantmbp3narlmt103and16kdamycobacteriumtuberculosisantigensinbraziliantuberculosispatients AT leiladesouzafonseca cellproliferationandinterferongammaresponsetorecombinantmbp3narlmt103and16kdamycobacteriumtuberculosisantigensinbraziliantuberculosispatients AT mariahelenaferessaad cellproliferationandinterferongammaresponsetorecombinantmbp3narlmt103and16kdamycobacteriumtuberculosisantigensinbraziliantuberculosispatients |
_version_ |
1725974116376248320 |