Recruitment dynamics of ESCRT-III and Vps4 to endosomes and implications for reverse membrane budding

Recruitment dynamics of ESCRT-III and Vps4 to endosomes and implications for reverse membrane budding

The ESCRT machinery mediates reverse membrane scission. By quantitative fluorescence lattice light-sheet microscopy, we have shown that ESCRT-III subunits polymerize rapidly on yeast endosomes, together with the recruitment of at least two Vps4 hexamers. During their 3–45 s lifetimes, the ESCRT-III...

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Bibliographic Details
Main Authors: Manuel Alonso Y Adell, Simona M Migliano, Srigokul Upadhyayula, Yury S Bykov, Simon Sprenger, Mehrshad Pakdel, Georg F Vogel, Gloria Jih, Wesley Skillern, Reza Behrouzi, Markus Babst, Oliver Schmidt, Michael W Hess, John AG Briggs, Tomas Kirchhausen, David Teis
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2017-10-01
Series:eLife
Subjects:
ESCRT
endosomes
membrane budding
lattice light sheet microscopy
3D tracking
electron tomography
Online Access:https://elifesciences.org/articles/31652

Internet

https://elifesciences.org/articles/31652

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