Improving the Identification and Coverage of Plant Transmembrane Proteins in Medicago Using Bottom–Up Proteomics

Improving the Identification and Coverage of Plant Transmembrane Proteins in Medicago Using Bottom–Up Proteomics

Plant transmembrane proteins (TMPs) are essential for normal cellular homeostasis, nutrient exchange, and responses to environmental cues. Commonly used bottom–up proteomic approaches fail to identify a broad coverage of peptide fragments derived from TMPs. Here, we used mass spectrometry (MS) to co...

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Bibliographic Details
Main Authors: Han Chung Lee, Adam Carroll, Ben Crossett, Angela Connolly, Amani Batarseh, Michael A. Djordjevic
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-12-01
Series:Frontiers in Plant Science
Subjects:
transmembrane protein
transmembrane domain
liquid chromatography
mass spectrometry
detergent-free purification
Medicago truncatula
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2020.595726/full

Internet

https://www.frontiersin.org/articles/10.3389/fpls.2020.595726/full

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