Comparison of CRISPR-Cas9/Cas12a Ribonucleoprotein Complexes for Genome Editing Efficiency in the Rice Phytoene Desaturase (OsPDS) Gene

Comparison of CRISPR-Cas9/Cas12a Ribonucleoprotein Complexes for Genome Editing Efficiency in the Rice Phytoene Desaturase (OsPDS) Gene

Abstract Background Delivery of CRISPR reagents into cells as ribonucleoprotein (RNP) complexes enables transient editing, and avoids CRISPR reagent integration in the genomes. Another technical advantage is that RNP delivery can bypass the need of cloning and vector construction steps. In this work...

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Bibliographic Details
Main Authors: Raviraj Banakar, Mollie Schubert, Michael Collingwood, Christopher Vakulskas, Alan L. Eggenberger, Kan Wang
Format: Article
Language:English
Published: SpringerOpen 2020-01-01
Series:Rice
Subjects:
CRISPR
Cas9
Cas12a
Rice
Ribonucleoproteins
Synthetic guide RNAs
Online Access:https://doi.org/10.1186/s12284-019-0365-z

Internet

https://doi.org/10.1186/s12284-019-0365-z

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