Efficient generation of GGTA1-deficient pigs by electroporation of the CRISPR/Cas9 system into in vitro-fertilized zygotes

Efficient generation of GGTA1-deficient pigs by electroporation of the CRISPR/Cas9 system into in vitro-fertilized zygotes

Abstract Background Xenoantigens are a major source of concern with regard to the success of interspecific xenografts. GGTA1 encodes α1,3-galactosyltransferase, which is essential for the biosynthesis of galactosyl-alpha 1,3-galactose, the major xenoantigen causing hyperacute rejection. GGTA1-modifi...

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Bibliographic Details
Main Authors: Fuminori Tanihara, Maki Hirata, Nhien Thi Nguyen, Osamu Sawamoto, Takeshi Kikuchi, Masako Doi, Takeshige Otoi
Format: Article
Language:English
Published: BMC 2020-08-01
Series:BMC Biotechnology
Subjects:
GGTA1
CRISPR/Cas9
Electroporation
In vitro fertilization
Pig
Online Access:http://link.springer.com/article/10.1186/s12896-020-00638-7

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http://link.springer.com/article/10.1186/s12896-020-00638-7

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