Programmable Base Editing of the Sheep Genome Revealed No Genome-Wide Off-Target Mutations

Programmable Base Editing of the Sheep Genome Revealed No Genome-Wide Off-Target Mutations

Since its emergence, CRISPR/Cas9-mediated base editors (BEs) with cytosine deaminase activity have been used to precisely and efficiently introduce single-base mutations in genomes, including those of human cells, mice, and crop species. Most production traits in livestock are induced by point mutat...

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Bibliographic Details
Main Authors: Shiwei Zhou, Bei Cai, Chong He, Ying Wang, Qiang Ding, Jiao Liu, Yao Liu, Yige Ding, Xiaoe Zhao, Guanwei Li, Chao Li, Honghao Yu, Qifang Kou, Wenzhi Niu, Bjoern Petersen, Tad Sonstegard, Baohua Ma, Yulin Chen, Xiaolong Wang
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-03-01
Series:Frontiers in Genetics
Subjects:
base editing
genome editing
point mutation
whole genome sequencing
off-target mutation
Online Access:https://www.frontiersin.org/article/10.3389/fgene.2019.00215/full

Internet

https://www.frontiersin.org/article/10.3389/fgene.2019.00215/full

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