Label‐Free Fluorescent Kinase and Phosphatase Enzyme Assays with Supramolecular Host‐Dye Pairs

Label‐Free Fluorescent Kinase and Phosphatase Enzyme Assays with Supramolecular Host‐Dye Pairs

Abstract The combination of the macrocyclic hosts p‐sulfonatocalix[4]arene and cucurbit[7]uril with the fluorescent dyes lucigenin and berberine affords two label‐free enzyme assays for the detection of kinase and phosphatase activity by fluorescence monitoring. In contrast to established assays, no...

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Bibliographic Details
Main Authors: Yan‐Cen Liu, Dr. Shu Peng, Lora Angelova, Prof. Dr. Werner M. Nau, Dr. Andreas Hennig
Format: Article
Language:English
Published: Wiley-VCH 2019-11-01
Series:ChemistryOpen
Subjects:
fluorescence
enzyme assay
host-guest chemistry
kinase
phosphatase
Online Access:https://doi.org/10.1002/open.201900299

Internet

https://doi.org/10.1002/open.201900299

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