Isolation, culture, and functional analysis of hepatocytes from mice with fatty liver disease

Isolation, culture, and functional analysis of hepatocytes from mice with fatty liver disease

Summary: We present a protocol for isolating hepatocytes from mice with established non-alcoholic fatty liver disease. This protocol consists of liver perfusion using a peristaltic pump, followed by a modified 25% and 90% Percoll gradient centrifugation protocol to capture lipid-laden hepatocytes th...

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Bibliographic Details
Main Authors: Yunshin Jung, Meng Zhao, Katrin J. Svensson
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Cell isolation
Single Cell
Metabolism
Model Organisms
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720302094

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http://www.sciencedirect.com/science/article/pii/S2666166720302094

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