Effect of Elderberry (Sambucus nigra L.) Extract Supplementation in STZ-Induced Diabetic Rats Fed with a High-Fat Diet
Elderberry (Sambucus nigra L.) lipophilic and polar extract dietary supplementation effects were evaluated according to diabetes management indices, using an in vivo model. A research pipeline was constructed, that ranged from extract preparation, partial chemical characterization and toxicity evalu...
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doaj-c05edb64cdd04de3852b9c141685466f2020-11-24T23:28:06ZengMDPI AGInternational Journal of Molecular Sciences1422-00672016-12-011811310.3390/ijms18010013ijms18010013Effect of Elderberry (Sambucus nigra L.) Extract Supplementation in STZ-Induced Diabetic Rats Fed with a High-Fat DietÂngelo C. Salvador0Ewelina Król1Virgínia C. Lemos2Sónia A. O. Santos3Fernanda P. M. S. Bento4Carina P. Costa5Adelaide Almeida6Dawid Szczepankiewicz7Bartosz Kulczyński8Zbigniew Krejpcio9Armando J. D. Silvestre10Sílvia M. Rocha11Organic Chemistry, Natural Products and Food Stuffs Research Unit, QOPNA, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalHuman Nutrition and Hygiene, Poznan University of Life Sciences, Wojska Polskiego 31, 60-637 Poznan, PolandOrganic Chemistry, Natural Products and Food Stuffs Research Unit, QOPNA, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalAveiro Institute of Materials, CICECO, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalOrganic Chemistry, Natural Products and Food Stuffs Research Unit, QOPNA, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalDepartment of Biology, University of Aveiro, 3810-193 Aveiro, PortugalCentre for Environmental and Marine Studies, CESAM, Department of Biology, University of Aveiro, 3810-193 Aveiro, PortugalDepartment of Animal Physiology and Biochemistry, Poznan University of Life Sciences, Wojska Polskiego 35, 60-637 Poznan, PolandHuman Nutrition and Hygiene, Poznan University of Life Sciences, Wojska Polskiego 31, 60-637 Poznan, PolandHuman Nutrition and Hygiene, Poznan University of Life Sciences, Wojska Polskiego 31, 60-637 Poznan, PolandAveiro Institute of Materials, CICECO, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalOrganic Chemistry, Natural Products and Food Stuffs Research Unit, QOPNA, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalElderberry (Sambucus nigra L.) lipophilic and polar extract dietary supplementation effects were evaluated according to diabetes management indices, using an in vivo model. A research pipeline was constructed, that ranged from extract preparation, partial chemical characterization and toxicity evaluation, to examining the elderberry extract dietary supplementation effects on biofluid and tissues. Extracts toxicity was screened using an Aliivibrio fischeri bioluminescence model. A concentration of up to 60 mg/L was selected, and rat doses for oral supplementation were computed applying the interspecies correlation between A. fischeri and rats. Wistar type 2 diabetic rats, induced by streptozotocin (STZ), were fed a high-fat diet and supplemented for 4 weeks at doses of 190 and 350 mg/kg body weight/day of lipophilic and polar extract, respectively. As far as we know, lipophilic elderberry extract supplementation was assessed for the first time, while polar extract was administrated at higher doses and for a shorter period compared to previous studies, aiming to evaluate subacute supplementation effects. The polar extract modulated glucose metabolism by correcting hyperglycemia, while the lipophilic extract lowered insulin secretion. Both extracts lowered insulin resistance, without remarkable alterations to hematological indices, sera lipids and sera and tissular trace element homeostasis. In conclusion, elderberries are a potential source of bioactive compounds for formulations to be used as co-adjuvants in diabetes management.http://www.mdpi.com/1422-0067/18/1/13diabetic ratselderberry extracts supplementationhigh-fat dietlipophilic extractpolar extractSambucus nigra L. |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ângelo C. Salvador Ewelina Król Virgínia C. Lemos Sónia A. O. Santos Fernanda P. M. S. Bento Carina P. Costa Adelaide Almeida Dawid Szczepankiewicz Bartosz Kulczyński Zbigniew Krejpcio Armando J. D. Silvestre Sílvia M. Rocha |
spellingShingle |
Ângelo C. Salvador Ewelina Król Virgínia C. Lemos Sónia A. O. Santos Fernanda P. M. S. Bento Carina P. Costa Adelaide Almeida Dawid Szczepankiewicz Bartosz Kulczyński Zbigniew Krejpcio Armando J. D. Silvestre Sílvia M. Rocha Effect of Elderberry (Sambucus nigra L.) Extract Supplementation in STZ-Induced Diabetic Rats Fed with a High-Fat Diet International Journal of Molecular Sciences diabetic rats elderberry extracts supplementation high-fat diet lipophilic extract polar extract Sambucus nigra L. |
author_facet |
Ângelo C. Salvador Ewelina Król Virgínia C. Lemos Sónia A. O. Santos Fernanda P. M. S. Bento Carina P. Costa Adelaide Almeida Dawid Szczepankiewicz Bartosz Kulczyński Zbigniew Krejpcio Armando J. D. Silvestre Sílvia M. Rocha |
author_sort |
Ângelo C. Salvador |
title |
Effect of Elderberry (Sambucus nigra L.) Extract Supplementation in STZ-Induced Diabetic Rats Fed with a High-Fat Diet |
title_short |
Effect of Elderberry (Sambucus nigra L.) Extract Supplementation in STZ-Induced Diabetic Rats Fed with a High-Fat Diet |
title_full |
Effect of Elderberry (Sambucus nigra L.) Extract Supplementation in STZ-Induced Diabetic Rats Fed with a High-Fat Diet |
title_fullStr |
Effect of Elderberry (Sambucus nigra L.) Extract Supplementation in STZ-Induced Diabetic Rats Fed with a High-Fat Diet |
title_full_unstemmed |
Effect of Elderberry (Sambucus nigra L.) Extract Supplementation in STZ-Induced Diabetic Rats Fed with a High-Fat Diet |
title_sort |
effect of elderberry (sambucus nigra l.) extract supplementation in stz-induced diabetic rats fed with a high-fat diet |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2016-12-01 |
description |
Elderberry (Sambucus nigra L.) lipophilic and polar extract dietary supplementation effects were evaluated according to diabetes management indices, using an in vivo model. A research pipeline was constructed, that ranged from extract preparation, partial chemical characterization and toxicity evaluation, to examining the elderberry extract dietary supplementation effects on biofluid and tissues. Extracts toxicity was screened using an Aliivibrio fischeri bioluminescence model. A concentration of up to 60 mg/L was selected, and rat doses for oral supplementation were computed applying the interspecies correlation between A. fischeri and rats. Wistar type 2 diabetic rats, induced by streptozotocin (STZ), were fed a high-fat diet and supplemented for 4 weeks at doses of 190 and 350 mg/kg body weight/day of lipophilic and polar extract, respectively. As far as we know, lipophilic elderberry extract supplementation was assessed for the first time, while polar extract was administrated at higher doses and for a shorter period compared to previous studies, aiming to evaluate subacute supplementation effects. The polar extract modulated glucose metabolism by correcting hyperglycemia, while the lipophilic extract lowered insulin secretion. Both extracts lowered insulin resistance, without remarkable alterations to hematological indices, sera lipids and sera and tissular trace element homeostasis. In conclusion, elderberries are a potential source of bioactive compounds for formulations to be used as co-adjuvants in diabetes management. |
topic |
diabetic rats elderberry extracts supplementation high-fat diet lipophilic extract polar extract Sambucus nigra L. |
url |
http://www.mdpi.com/1422-0067/18/1/13 |
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