Procedural Requirements and Recommendations for Multiplex Immunofluorescence Tyramide Signal Amplification Assays to Support Translational Oncology Studies

Procedural Requirements and Recommendations for Multiplex Immunofluorescence Tyramide Signal Amplification Assays to Support Translational Oncology Studies

In the development of a multiplex immunofluorescence (IF) platform and the optimization and validation of new multiplex IF panels using a tyramide signal amplification system, several technical requirements are important for high-quality staining, analysis, and results. The aim of this review is to...

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Bibliographic Details
Main Authors: Edwin Roger Parra, Mei Jiang, Luisa Solis, Barbara Mino, Caddie Laberiano, Sharia Hernandez, Swati Gite, Anuj Verma, Michael Tetzlaff, Cara Haymaker, Auriole Tamegnon, Jaime Rodriguez-Canales, Clifford Hoyd, Chantale Bernachez, Ignacio Wistuba
Format: Article
Language:English
Published: MDPI AG 2020-01-01
Series:Cancers
Subjects:
tumor immune profiling
multiplex immunofluorescence
tyramide signal amplification
image analysis
Online Access:https://www.mdpi.com/2072-6694/12/2/255

Internet

https://www.mdpi.com/2072-6694/12/2/255

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