Simple innovative adaptor to improve genome walking with convenient PCR

Simple innovative adaptor to improve genome walking with convenient PCR

Abstract Background Various polymerase chain reaction (PCR)-based methods have been applied for the development of genome walking (GW) technique. These methods which could be based on the application of restriction enzymes or primers have various efficiencies to identify the unknown nucleotide seque...

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Bibliographic Details
Main Authors: Seyedeh-Samira Ashrafmansouri, Hossein Kamaladini, Fatemeh Haddadi, Marie Seidi
Format: Article
Language:English
Published: SpringerOpen 2020-10-01
Series:Journal of Genetic Engineering and Biotechnology
Subjects:
Genome walking
Adaptor
Restriction enzymes
PCR
map30 gene
pTZ57R plasmid
Online Access:http://link.springer.com/article/10.1186/s43141-020-00082-2

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http://link.springer.com/article/10.1186/s43141-020-00082-2

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